Anti-CDK1 (phospho T14)抗体(ab58509)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T14)
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CDK1 (phospho T14)抗体
参阅全部 CDK1 一抗 -
描述
兔多克隆抗体to CDK1 (phospho T14) -
宿主
Rabbit -
特异性
ab58509 peptide sequence is 100% identical to the sequence of CDK1 and CDK2. Therefore, this antibody is predicted to recognize both proteins.
Detects endogenous levels of CDC2 only when phosphorylated at threonine 14.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human CDK1 aa 1-100 (phospho T14).
Database link: P06493 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58509于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa). |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
组织特异性
Isoform 2 is found in breast cancer tissues. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
- SwissProt: P06493 Human
- SwissProt: P11440 Mouse
- SwissProt: P39951 Rat
- Unigene: 334562 Human
see all -
形式
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
别名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
图片
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Western blot - Anti-CDK1 (phospho T14) antibody (ab58509)
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM for 30 mins, using CDK1 (phospho T14) Antibody (ab58509). The lane on the right is blocked with the phospho peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-CDK1 (phospho T14) antibody (ab58509)Immunofluorescence analysis of COS7 cells using ab58509 at 1/1000 dilution.
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Western blot - Anti-CDK1 (phospho T14) antibody (ab58509)All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins)
Lane 2 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins), plus immunising peptide
Predicted band size: 34 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab58509 被引用在 7 文献中.
- Benada J et al. Synthetic lethal interaction between WEE1 and PKMYT1 is a target for multiple low-dose treatment of high-grade serous ovarian carcinoma. NAR Cancer 5:zcad029 (2023). PubMed: 37325550
- Szychowski J et al. Discovery of an Orally Bioavailable and Selective PKMYT1 Inhibitor, RP-6306. J Med Chem 65:10251-10284 (2022). PubMed: 35880755
- Gallo D et al. CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition. Nature 604:749-756 (2022). PubMed: 35444283
- Tanaka H et al. HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology. Commun Biol 4:1175 (2021). PubMed: 34635772
- Homma H et al. DNA damage in embryonic neural stem cell determines FTLDs' fate via early-stage neuronal necrosis. Life Sci Alliance 4:N/A (2021). PubMed: 34130995
- Jin H et al. A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. Sci Adv 6:eaba3418 (2020). PubMed: 32426509
- Chow JP & Poon RY The CDK1 inhibitory kinase MYT1 in DNA damage checkpoint recovery. Oncogene : (2012). PubMed: 23146904