重组Anti-CDK1抗体[EPR165] (ab133327)
![Western blot - Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-146850-anti-cdk1-antibody-epr165-western-blot.jpg "Western blot - Anti-CDK1 antibody [EPR165] (ab133327)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR165] to CDK1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CDK1抗体[EPR165]
参阅全部 CDK1 一抗 -
描述
兔单克隆抗体[EPR165] to CDK1 -
宿主
Rabbit -
特异性
We have preliminary internal testing data to indicate this antibody doesn't react with CDK2, CDK3 and CDK5. Please contact us for more information.
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经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CDK1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P06493 -
阳性对照
- WB: HeLa, Saos-2, Namalwa, and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. IP: HeLa. Flow Cyt: Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR165 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133327于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/130.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (1) |
1/100 - 1/250.
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| WB | (2) |
1/10000 - 1/50000. Predicted molecular weight: 34 kDa.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/100 - 1/250. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 34 kDa. |
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IP
1/10 - 1/100. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
组织特异性
Isoform 2 is found in breast cancer tissues. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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形式
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
别名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
图片
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Western blot - Anti-CDK1 antibody [EPR165] (ab133327)All lanes : Anti-CDK1 antibody [EPR165] (ab133327) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : Namalwa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-275745-anti-cdk1-antibody-epr165-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDK1 antibody [EPR165] (ab133327)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 with ab133327 at 1/500 dilution (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). DAPI nuclear counterstain. Confocal image showing strong nuclear and weakly cytoplasmic staining on HeLa cell line.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-146849-anti-cdk1-antibody-epr165-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [EPR165] (ab133327)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling CDK1 with ab133327 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-269711-anti-cdk1-antibody-epr165-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (ab133327)Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CDK1 with purified ab133327 at 1/130 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Immunoprecipitation - Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-7-anti-cdk1-antibody-epr165-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-CDK1 antibody [EPR165] (ab133327)CDK1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 133327 at 1/20 dilution (2µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab133327 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133327 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-CDK1 antibody [EPR165] (ab133327)](https://www.abcam.cn/ps/products/133/ab133327/Images/ab133327-5-benefits-of-recombinant-antibodies.png)
Anti-CDK1 antibody [EPR165] (ab133327)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (85)
ab133327 被引用在 85 文献中.
- Yanaihara N et al. Paclitaxel sensitizes homologous recombination-proficient ovarian cancer cells to PARP inhibitor via the CDK1/BRCA1 pathway. Gynecol Oncol 168:83-91 (2023). PubMed: 36403366
- Doi K et al. PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes. FEBS J 290:745-762 (2023). PubMed: 36047562
- Zhu S et al. CDK1 bridges NF-κB and β-catenin signaling in response to H. pylori infection in gastric tumorigenesis. Cell Rep 42:112005 (2023). PubMed: 36681899
- Chen J et al. Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer. Biomed Res Int 2023:1733100 (2023). PubMed: 36718148
- Liu J et al. GRIK3 deficiency promotes non-small cell lung cancer progression by the regulation of the UBE2C/CDK1/Wnt signaling pathway. Am J Cancer Res 13:2066-2075 (2023). PubMed: 37293152