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重组RabMAb

重组Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15)抗体[EPR2233Y] (ab76146)

Reviews (2)Q&A (1)References (27)
Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Western blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Dot Blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Immunoprecipitation - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
  • Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2233Y] to CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15)
  • Suitable for: IHC-P, Dot blot, WB, IP
  • Reacts with: Rat, Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15)抗体[EPR2233Y]

  • 描述

    兔单克隆抗体[EPR2233Y] to CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15)

  • 宿主

    Rabbit

  • 特异性

    This antibody detects Cdk1, 2, 3, 5 phosphorylated on tyrosine 15.

  • 经测试应用

    适用于: IHC-P, Dot blot, WB, IPmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Rat, Human, Recombinant fragment
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human CDK2 (phospho Y15). The exact sequence is proprietary.

  • 阳性对照

    • WB: HeLa cell and mouse spleen tissue lysates; 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged blank expression vector, whole cell lysate; 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK1/2/3/5 expression vector, whole cell lysate IHC-P: Human transitional cell carcinoma of bladder and rat colon tissues. IP: HeLa whole cell lysate (ab150035).

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab76146于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Dot blot
1/1000.
WB (2)
1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

A band of 65 kDa is also observed in 293T cells overexpressing cdk1, 2, 3, 5 (phospho Y15)
IP
1/100.

For unpurified use at 1/50.
说明
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Dot blot
1/1000.
WB
1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 34 kDa).

For unpurified use at 1/1000 - 1/10000.

A band of 65 kDa is also observed in 293T cells overexpressing cdk1, 2, 3, 5 (phospho Y15)
IP
1/100.

For unpurified use at 1/50.
应用说明
Is unsuitable for ICC/IF.

靶标

图片

  • Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Western blot - Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/50000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, treated with alkaline phosphatase for 1hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa


    Exposure time: 10 seconds


    Blocking/diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Western blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    All lanes : Anti-CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15) antibody [EPR2233Y] (ab76146) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged blank expression vector, whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK1 expression vector, whole cell lysate
    Lane 3 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK1 expression vector, whole cell lysate. Then the membrane was incubated with phosphatase
    Lane 4 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK2 expression vector, whole cell lysate
    Lane 5 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK2 expression vector, whole cell lysate. Then the membrane was incubated with phosphatase
    Lane 6 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK3 expression vector, whole cell lysate
    Lane 7 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK3 expression vector, whole cell lysate. Then the membrane was incubated with phosphatase
    Lane 8 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK5 expression vector, whole cell lysate
    Lane 9 : 293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CDK5 expression vector, whole cell lysate. Then the membrane was incubated with phosphatase

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34,65 kDa why is the actual band size different from the predicted?



    ab76146 detects Cdk1, 2, 3, 5 phosphorylated on tyrosine 15.

  • Dot Blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Dot Blot - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

    Dot blot analysis of Cdk2 (pY15) peptide (Lane 1) and Cdk2 non-phospho peptide (Lane 2) labelling Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) with purified ab76146 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Immunoprecipitation - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

    ab76146 (purified) at 1/100 immunoprecipitating Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) in HeLa cell lysates (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) with purified ab76146 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. A prediluted HRP polymer-conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)
    Anti-Cdk1 + Cdk2 + Cdk3 + Cdk5 (phospho Y15) antibody [EPR2233Y] (ab76146)

文献 (27)

发表研究结果有使用 ab76146？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab76146 被引用在 27 文献中.

  • Lu X  et al. Testis developmental related gene 1 promotes non-small-cell lung cancer through the microRNA-214-5p/Krüppel-like factor 5 axis. Bioengineered 13:603-616 (2022). PubMed: 34856848
  • Gray S  et al. Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. Cell Rep 32:107858 (2020). PubMed: 32640224
  • Nam GH  et al. The peptide AC 2 isolated from Bacillus-treated Trapa japonica fruit extract rescues DHT (dihydrotestosterone)-treated human dermal papilla cells and mediates mTORC1 signaling for autophagy and apoptosis suppression. Sci Rep 9:16903 (2019). PubMed: 31729428
  • Diaz-Flores E  et al. Bcl-2 Is a Therapeutic Target for Hypodiploid B-Lineage Acute Lymphoblastic Leukemia. Cancer Res 79:2339-2351 (2019). PubMed: 30862722
  • Qi W  et al. Inhibition of Wee1 sensitizes AML cells to ATR inhibitor VE-822-induced DNA damage and apoptosis. Biochem Pharmacol 164:273-282 (2019). PubMed: 31014753
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Cdk2 (phospho Y15) antibody [EPR2233Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Treatment
2 ´M MK-1775
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Remi Buisson

Verified customer

提交于 Oct 27 2014

Western blot abreview for Anti-Cdk2 (phospho Y15) antibody [EPR2233Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (osteosarcoma cells)
Specification
osteosarcoma cells
Treatment
lane 1 control, lane 2 siRNA for CDK2, 48h
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sonia Rocha

Verified customer

提交于 Sep 26 2014

Question

I am very interested in antibodies that might discriminate between phospho-CDK1 and phospho-CDK2. ab76146 is described as detecting CDK2 phospho-Y15, but your site does not say whether it also detects CDK1 phosphorylated at this site (which is the same consensus sequence). Thanks.

Read More
Answer

Thank you for your inquiry. I have confirmed with the laboratory that we have not tested cross-reactivity with phospho-CDK1. Sequence analysis shows that the immunogen of ab76146 shares 100% identity with human CDK1. Thus, I would predict cross-reactivity, but it is not guaranteed without data.

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