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Anti-CDCP1抗体(ab1377)

Submit a review Q&A (4)References (15)
Western blot - Anti-CDCP1 antibody (ab1377)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)

Key features and details

  • Goat polyclonal to CDCP1
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-CDCP1 antibody [EPR23162-102] (ab252947)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-CDCP1抗体
    参阅全部 CDCP1 一抗

  • 描述

    山羊多克隆抗体to CDCP1

  • 宿主

    Goat

  • 特异性

    This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.

  • 经测试应用

    适用于: IHC-P, IP, ICC/IF, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human CDCP1 aa 800 to the C-terminus (C terminal).
    Database link: NP_073753.3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1377于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. PubMed: 16823897
IP
Use at an assay dependent concentration. PubMed: 17785447
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).

1 hour primary incubation is recommended for this product.
说明
IHC-P
Use at an assay dependent concentration. PubMed: 16823897
IP
Use at an assay dependent concentration. PubMed: 17785447
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.

  • 组织特异性

    Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

  • 序列相似性

    Contains 1 CUB domain.

  • 翻译后修饰

    Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

  • 细胞定位

    Secreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 9030022E12Rik antibody
      • AA409659 antibody
      • CD 318 antibody
      • CD_antigen=CD318 antibody
      • CD318 antibody
      • CD318 antigen antibody
      • CDCP 1 antibody
      • Cdcp1 antibody
      • CDCP1_HUMAN antibody
      • CUB domain containing protein 1 antibody
      • CUB domain-containing protein 1 antibody
      • E030027H19Rik antibody
      • FLJ13772 antibody
      • FLJ22375 antibody
      • FLJ22969 antibody
      • Membrane glycoprotein gp140 antibody
      • MGC31813 antibody
      • OTTHUMP00000164120 antibody
      • OTTHUMP00000209568 antibody
      • RGD1305578 antibody
      • SIMA 135 antibody
      • SIMA135 antibody
      • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
      • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
      • Transmembrane and associated with src kinases antibody
      • TRASK antibody
      see all

    图片

    • Western blot - Anti-CDCP1 antibody (ab1377)
      Western blot - Anti-CDCP1 antibody (ab1377)
      ab1377 (1 µg/ml) staining CDCP1 human colon whole cell lysate (35 µg) in RIPA buffer. Incubation was for 1 hour and detection was by chemiluminescence.
    • Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)
      Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)
      ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献 (15)

    发表研究结果有使用 ab1377？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab1377 被引用在 15 文献中.

    • Zhang F  et al. Methylation of microRNA-338-5p by EED promotes METTL3-mediated translation of oncogene CDCP1 in gastric cancer. Aging (Albany NY) 13:12224-12238 (2021). PubMed: 33882457
    • Lin YS  et al. Resveratrol-mediated ADAM9 degradation decreases cancer progression and provides synergistic effects in combination with chemotherapy. Am J Cancer Res 10:3828-3837 (2020). PubMed: 33294270
    • Huang L  et al. CUB Domain?-Containing Protein?-1 Promotes Proliferation, Migration and Invasion in Cervical Cancer Cells. Cancer Manag Res 12:3759-3769 (2020). PubMed: 32547212
    • Wright HJ  et al. CDCP1 cleavage is necessary for homodimerization-induced migration of triple-negative breast cancer. Oncogene 35:4762-72 (2016). IF . PubMed: 26876198
    • Chiu KL  et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis. Sci Rep 5:16426 (2015). PubMed: 26553452
    View all Publications for this product

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Question

    Csutomer kindly called to inquire about our CDCP1 antibodies, quality control data and use in FACS.

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    Answer

    Thank you for contacting us.

    I have been able to attain immunogen data used to create the product ab118268 for you which has been created using the full length recombinant protein. I am still awaiting further data including any additional quality control or information about used of these products in FACs.



    While we cannot be sure of the region to which these product bind, for UNTESTED species and/or applications, sucgh as FACs with these products, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    This antibody is a fast track. Can I provide details of image and what "low signal" refers to when tested against Human Liver and Brain lysates.

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    Answer

    Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that this antibody was tested by western blotting using standard tissues/cells, on this occassion human liver and brain lysates. No bands were observed and the general background was low. It is not known whether this protein is expressed in these cells. I am sorry that I cannot be of more assistance.

    Read More

    Question

    I request some information about your anti-gamma tubulin antibodies. I would like to know, in as much detail as possible, about what antigen the antibodies (ab1377, ab11318, ab11320, ab11321) directed against gamma tubulin were raised. Many of the antibodies on the market were raised against peptide 38-53 of human gamma tubulin. Were any of your antibodies raised against a different portion of the gamma tubulin protein, or against a full length gamma tubulin? Are you at liberty to disclose who (which laboratory) produced each antibody originally? Thank you,

    Read More
    Answer

    Thank you for your enquiry. Details of the immunogens used for each of the antisera that you are interested in are as follows: I think that you may have mistyped the first antiserum as it is not directed against tubulin. CDCP1 antibody (ab1377) - Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1. Gamma Tubulin antibody - Centrosome Marker (ab11318) - Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11320) - Synthetic peptide: DEYHAATRPDYISWGTQEQ corresponding to amino acids 433-451 of gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11321) Synthetic peptide: AATRPDYISWGTQEQ corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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    Question

    Do you have a sample antibody program? I am really tired of buying Abs that don't work. At least you are forward enough to state that it hasn't worked as yet on the tested samples. If you could send me a free trial amount of the antibody and it works, I will order a full aliquot. I am also in a position to test your Ab, as I have monoclonal antibodies to CDCP1 that do work - too well, in fact. My antibodies were made with a peptide sequence that probably contains a sequence conserved in multiple proteins since they are recognizing multiple proteins. Moreover, all protein interactions can be inhibited by immunogen peptide but not an irrelevant peptide. Anyway, your antibody would potentially allow me to define specific CDCP1 interactions, and I would be able to provide you with information on your antibody reactivity. Please let me know if this is a mutually agreeable avenue. Thanks.

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    Answer

    I am afraid that I am unable to offer you a trial sample of this antibody for preliminary testing. The antibody is priced to reflect the fact that it is investigative grade and all the information we have is on the datasheet.

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