重组Anti-Cdc6抗体[EPR714(2)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdc6 with Purified ab109315 at 1 : 100 dilution (9.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Cdc6 with Purified ab109315 at 1 : 90 dilution (10.4 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

• ICC/IF

AbReview60916****

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Unpurified ab109315 staining Cdc6 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 (1/200) was used as the secondary antibody.

This image is courtesy of an abreview by Kirk Mcmanus

• WB

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Western blot - Anti-Cdc6 antibody [EPR714(2)] (AB109315)

Blocking and diluting buffer : 5% NFDM/TBST.

Hydroxyurea induces Cdc6 expression (PMID : 11346650)

All lanes:

Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 0.5 µg/mL

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) treated with 20mM Hydroxyurea for 24 hours whole cell lysates at 15 µg

Lane 3:

HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 4:

HCT116 (Human colorectal carcinoma epithelial cell) treated with 1mM Hydtoxyurea for 16 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

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