Anti-CDC42 Binding蛋白Kinase Beta抗体(ab226345)
Key features and details
- Rabbit polyclonal to CDC42 Binding Protein Kinase Beta
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-CDC42 Binding蛋白Kinase Beta抗体 -
描述
兔多克隆抗体to CDC42 Binding蛋白Kinase Beta -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
-
免疫原
Synthetic peptide within Human CDC42 Binding Protein Kinase Beta aa 750-800. The exact sequence is proprietary.
Database link: Q9Y5S2 -
阳性对照
- WB: HeLa and HEK-293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226345 was affinity purified using an epitope specific to CDC42 Binding Protein Kinase Beta immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226345于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 194 kDa.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 194 kDa. |
靶标
-
功能
May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation. -
组织特异性
Expressed in all tissues examined, with high levels in heart, brain, placenta and lung. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. - Information by UniProt
-
数据库链接
- Entrez Gene: 453180 Chimpanzee
- Entrez Gene: 508226 Cow
- Entrez Gene: 490868 Dog
- Entrez Gene: 9578 Human
- Entrez Gene: 217866 Mouse
- Entrez Gene: 100457144 Orangutan
- Entrez Gene: 100156588 Pig
- Entrez Gene: 100355493 Rabbit
see all -
别名
- CDC42 binding protein kinase beta antibody
- CDC42-binding protein kinase beta antibody
- CDC42BPB antibody
see all
图片
-
Western blot - Anti-CDC42 Binding Protein Kinase Beta antibody (ab226345)All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab226345) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 194 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab226345 尚未被引用在任何文献中。