Anti-CDC42抗体[M152] (ab41429)
Key features and details
- Mouse monoclonal [M152] to CDC42
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
-
产品名称
Anti-CDC42抗体[M152]
参阅全部 CDC42 一抗 -
描述
小鼠单克隆抗体[M152] to CDC42 -
宿主
Mouse -
特异性
The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant CDC42 (Rat)
-
阳性对照
- WB: MCF7, HepG2, HAP1 and Jurkat cell lysate; Mouse brain lysate. ICC/IF: SH-SY5Y cells.
-
常规说明
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
M152 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41429于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/50.
|
|
WB | (3) |
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
说明 |
---|
ICC/IF
1/50. |
WB
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
靶标
-
功能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
翻译后修饰
AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 64465 Rat
- Omim: 116952 Human
- SwissProt: P60953 Human
- SwissProt: P60766 Mouse
- SwissProt: Q8CFN2 Rat
- Unigene: 467637 Human
see all -
形式
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
别名
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
图片
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.ab41429 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
-
Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilution
Lanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lanes 1-2 : human jurkat cells
Lanes 3-4 : mouse brain
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)Image from Haebig K et al, PLoS One. 2010 Oct 29;5(10):e13762, Fig 2. doi:10.1371/journal.pone.0013762
ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence. SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.
-
All lanes : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lane 1 : Rabbit heart tissue lysate at 10 µg
Lane 2 : Rabbit heart tissue lysate at 20 µg
Lane 3 : Rabbit heart tissue lysate at 40 µg
Lane 4 : Rabbit heart tissue lysate at 60 µg
Lane 5 : Rabbit heart tissue lysate at 80 µg
Lane 6 : Rabbit heart tissue lysate at 100 µg
Secondary
All lanes : Donkey anti-mouse IgG (HRP) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
-
PC-12 (Rat adrenal gland pheochromocytoma cell line) cells grown for 4 days on poly-D-lysine-coated plates in the rpesence (200 ng/ml) or absence (control) of Nerve Growth Factor (NGF) labeling CDC42 with ab41429 in ICC/IF. Primary antibody was used at 1/50 dilution followed by a donkey anti-mouse secondaty antibody (Cy2).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab41429 被引用在 16 文献中.
- Amoah A et al. Aging of human hematopoietic stem cells is linked to changes in Cdc42 activity. Haematologica 107:393-402 (2022). PubMed: 33440922
- Heib T et al. RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes. Cell Rep 35:109102 (2021). PubMed: 33979620
- Zhou Y et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver. Theranostics 11:7262-7275 (2021). PubMed: 34158849
- López-Ureña D et al. Toxin B Variants from Clostridium difficile Strains VPI 10463 and NAP1/027 Share Similar Substrate Profile and Cellular Intoxication Kinetics but Use Different Host Cell Entry Factors. Toxins (Basel) 11:N/A (2019). PubMed: 31212980
- Zhang Q et al. miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42. Oncol Lett 16:3101-3107 (2018). PubMed: 30127901