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重组RabMAb

重组Anti-CDC40抗体[EPR12539] (ab175924)

Submit a review Submit a question References (2)
Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
  • Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (ab175924)
  • Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
  • Anti-CDC40 antibody [EPR12539] (ab175924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12539] to CDC40
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CDC40抗体[EPR12539]
    参阅全部 CDC40 一抗

  • 描述

    兔单克隆抗体[EPR12539] to CDC40

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human CDC40 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60508

  • 阳性对照

    • HeLa, K562, 293T, HUVEC cell lysates, colon tissue, transitional cell carcinoma tissue, HeLa and K562 Kcells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175924于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
应用说明
Is unsuitable for IP.

靶标

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (ab175924)
    Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (ab175924)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

     

     

  • Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
    Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
    All lanes : Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa

  • Anti-CDC40 antibody [EPR12539] (ab175924)
    Anti-CDC40 antibody [EPR12539] (ab175924)

文献 (2)

发表研究结果有使用 ab175924？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab175924 被引用在 2 文献中.

  • Kim JJ  et al. Dynamics of TERT regulation via alternative splicing in stem cells and cancer cells. PLoS One 18:e0289327 (2023). PubMed: 37531400
  • Chai G  et al. Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly. Neuron 109:241-256.e9 (2021). PubMed: 33220177

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