重组Anti-Cdc37抗体[EPR4935] (ab108305)
![Western blot - Anti-Cdc37 antibody [EPR4935] (ab108305)](https://www.abcam.cn/ps/products/108/ab108305/Images/ab108305-120827-anti-cdc37-antibody-epr4935-western-blot.jpg "Western blot - Anti-Cdc37 antibody [EPR4935] (ab108305)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4935] to Cdc37
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Cdc37抗体[EPR4935]
参阅全部 Cdc37 一抗 -
描述
兔单克隆抗体[EPR4935] to Cdc37 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- K562 cell lysate, Jurkat cell lysate, LnCaP cell lysate, SW480 cell lysate, human brain tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4935 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108305于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000 - 1/200000. Predicted molecular weight: 44 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
perform antigen retrieval |
| 说明 |
|---|
|
WB
1/50000 - 1/200000. Predicted molecular weight: 44 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. perform antigen retrieval |
靶标
-
功能
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
序列相似性
Belongs to the CDC37 family. -
翻译后修饰
Constitutively sumoylated by UBE2I. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 11140 Human
- Omim: 605065 Human
- SwissProt: Q16543 Human
- Unigene: 160958 Human
-
别名
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
图片
-
Western blot - Anti-Cdc37 antibody [EPR4935] (ab108305)All lanes : Anti-Cdc37 antibody [EPR4935] (ab108305) at 1/50000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc37 antibody [EPR4935] (ab108305)](https://www.abcam.cn/ps/products/108/ab108305/Images/ab108305-120826-anti-cdc37-antibody-epr4935-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc37 antibody [EPR4935] (ab108305)ab108305, at 1/250, staining Cdc37 in human brain tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Cdc37 antibody [EPR4935] (ab108305)](https://www.abcam.cn/ps/products/108/ab108305/Images/ab108305-3-benefits-of-recombinant-antibodies.png)
Anti-Cdc37 antibody [EPR4935] (ab108305)
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab108305 被引用在 1 文献中.
- Tirosh O et al. The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions. PLoS Pathog 11:e1005288 (2015). WB . PubMed: 26599541