重组Anti-Cdc37抗体[EPR4934] (ab109419)
![Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)](https://www.abcam.cn/ps/products/109/ab109419/Images/ab109419-396920-anti-cdc37-antibody-epr4934-westernblot-human-mouse-rat.jpg "Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4934] to Cdc37
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc37抗体[EPR4934]
参阅全部 Cdc37 一抗 -
描述
兔单克隆抗体[EPR4934] to Cdc37 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat, A431, K-562, LNCaP, NIH/3T3, Raw 264.7, PC-12, and C6 whole cell lysates. Rat and mouse heart tissue lysates; IP: Jurkat whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4934 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa).
|
|
| IP |
1/20.
For unpurified use at 1/10 - 1/100. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa). |
|
IP
1/20. For unpurified use at 1/10 - 1/100. |
靶标
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功能
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. -
序列相似性
Belongs to the CDC37 family. -
翻译后修饰
Constitutively sumoylated by UBE2I. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 11140 Human
- Entrez Gene: 12539 Mouse
- Entrez Gene: 114562 Rat
- Omim: 605065 Human
- SwissProt: Q16543 Human
- SwissProt: Q61081 Mouse
- SwissProt: Q63692 Rat
- Unigene: 160958 Human
see all -
别名
- CDC 37 antibody
- Cdc37 antibody
- CDC37 cell division cycle 37 homolog antibody
see all
图片
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Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)All lanes : Anti-Cdc37 antibody [EPR4934] (ab109419) at 1/10000 dilution (purified)
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 5 : Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6 : Mouse heart lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 9 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
![Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)](https://www.abcam.cn/ps/products/109/ab109419/Images/ab109419-396919-anti-cdc37-antibody-epr4934-westernblot-jurkat-human.jpg)
Western blot - Anti-Cdc37 antibody [EPR4934] (ab109419)Anti-Cdc37 antibody [EPR4934] (ab109419) at 1/10000 dilution (purified) + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
![Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (ab109419)](https://www.abcam.cn/ps/products/109/ab109419/Images/ab109419-3-anti-cdc37-antibody-epr4934-immunoprecipitation-jurkat-human.jpg)
Immunoprecipitation - Anti-Cdc37 antibody [EPR4934] (ab109419)Purified ab109419 at 1/20 dilution (0.5 µg) immunoprecipitating Cdc37 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10 µg
Lane 2 (+): ab109419 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109419 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 44 kDa -
![Anti-Cdc37 antibody [EPR4934] (ab109419)](https://www.abcam.cn/ps/products/109/ab109419/Images/ab109419-6-benefits-of-recombinant-antibodies.png)
Anti-Cdc37 antibody [EPR4934] (ab109419)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab109419 被引用在 2 文献中.
- Zang M et al. Cdc37 suppression induces plasma cell immaturation and bortezomib resistance in multiple myeloma via Xbp1s. Oncogenesis 9:31 (2020). PubMed: 32139666
- Synoradzki K & Bieganowski P Middle domain of human Hsp90 isoforms differentially binds Aha1 in human cells and alters Hsp90 activity in yeast. Biochim Biophys Acta 1853:445-52 (2015). PubMed: 25486457