重组Anti-Cdc27抗体[EPR7798] (ab129085)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7798] to Cdc27
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc27抗体[EPR7798]
参阅全部 Cdc27 一抗 -
描述
兔单克隆抗体[EPR7798] to Cdc27 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, 293T, and HeLa whole cell lysate (ab150035)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7798 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129085于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa). |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Entrez Gene: 360643 Rat
- Omim: 116946 Human
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
- SwissProt: Q4V8A2 Rat
- Unigene: 463295 Human
see all -
别名
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
图片
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All lanes : Anti-Cdc27 antibody [EPR7798] (ab129085) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 91 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab129085 被引用在 4 文献中.
- Ferragut Cardoso AP et al. miR-186 induces tetraploidy in arsenic exposed human keratinocytes. Ecotoxicol Environ Saf 256:114823 (2023). PubMed: 36989553
- Luong XG et al. Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption. Nucleic Acids Res 48:3257-3276 (2020). PubMed: 31970406
- Wu J et al. Overexpression of hsa-miR-186 induces chromosomal instability in arsenic-exposed human keratinocytes. Toxicol Appl Pharmacol 378:114614 (2019). PubMed: 31176655
- S Pedersen R et al. Profiling DNA damage response following mitotic perturbations. Nat Commun 7:13887 (2016). PubMed: 27976684