Anti-Cdc27抗体[AF3.1] (ab10538)
![Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.cn/ps/products/10/ab10538/Images/ab10538-18174-anti-cdc27-antibody-af31-western-blot.jpg "Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)"){kind=small}
Key features and details
- Mouse monoclonal [AF3.1] to Cdc27
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-Cdc27抗体[AF3.1]
参阅全部 Cdc27 一抗 -
描述
小鼠单克隆抗体[AF3.1] to Cdc27 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to amino acids 814-823 of Human Cdc27.
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阳性对照
- HeLa cell nuclear extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AF3.1 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10538于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 92 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Omim: 116946 Human
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
- Unigene: 463295 Human
- Unigene: 89845 Mouse
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别名
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
图片
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Western blot - Anti-Cdc27 antibody [AF3.1] (ab10538)All lanes : Anti-Cdc27 antibody [AF3.1] (ab10538)
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Mouse myeloid cell lysate
Predicted band size: 92 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
We have no more information on how this image was prepared by the academic who produced the antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.cn/ps/products/10/ab10538/Images/ab10538-18173-anti-cdc27-antibody-af31-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc27 antibody [AF3.1] (ab10538)IHC image of ab10538 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10538, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)](https://www.abcam.cn/ps/products/10/ab10538/Images/ab10538-18172-anti-cdc27-antibody-af31-flow-cytometry.jpg)
Flow Cytometry - Anti-Cdc27 antibody [AF3.1] (ab10538)Overlay histogram showing K562 cells stained with ab10538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab10538 被引用在 12 文献中.
- Chang CL et al. Prognostic and clinical significance of subcellular CDC27 for patients with rectal adenocarcinoma treated with adjuvant chemotherapy. Oncol Lett 24:238 (2022). PubMed: 35720473
- Villarroya-Beltri C et al. Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility. Sci Adv 8:eabq5914 (2022). PubMed: 36322655
- Tanno N et al. Phosphorylation of the Anaphase Promoting Complex activator FZR1/CDH1 is required for Meiosis II entry in mouse male germ cell. Sci Rep 10:10094 (2020). PubMed: 32572094
- Wu Q et al. APC/C-CDH1-Regulated IDH3ß Coordinates with the Cell Cycle to Promote Cell Proliferation. Cancer Res 79:3281-3293 (2019). PubMed: 31053633
- Link LA et al. PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Mol Cancer Res 14:634-46 (2016). PubMed: 27102006