Anti-Cdc27抗体(ab72214)
Key features and details
- Rabbit polyclonal to Cdc27
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdc27抗体
参阅全部 Cdc27 一抗 -
描述
兔多克隆抗体to Cdc27 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 225 and 275 of human Cdc27 (NP_001247.2).
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阳性对照
- Whole cell lysate from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72214于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 92 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC3/CDC27 family.
Contains 9 TPR repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 540660 Cow
- Entrez Gene: 996 Human
- Entrez Gene: 217232 Mouse
- Entrez Gene: 360643 Rat
- Omim: 116946 Human
- SwissProt: A7Z061 Cow
- SwissProt: P30260 Human
- SwissProt: A2A6Q5 Mouse
see all -
别名
- ANAPC3 antibody
- Anaphase Promoting Complex 3 antibody
- Anaphase promoting complex protein 3 antibody
see all
图片
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Western blot - Anti-Cdc27 antibody (ab72214)All lanes : Anti-Cdc27 antibody (ab72214) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 50 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-Cdc27 antibody (ab72214)Detection of Human Cdc27 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72214 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab72214 被引用在 1 文献中.
- Ren YQ et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015). WB ; Human . PubMed: 25943633