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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases

Anti-Cdc25A抗体(ab989)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (11)

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Western blot - Anti-Cdc25A antibody (ab989)
  • Immunoprecipitation - Anti-Cdc25A antibody (ab989)

Key features and details

  • Rabbit polyclonal to Cdc25A
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Recombinant human Cdc25A protein (ab90763)

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概述

  • 产品名称

    Anti-Cdc25A抗体
    参阅全部 Cdc25A 一抗
  • 描述

    兔多克隆抗体to Cdc25A
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).

  • 阳性对照

    • WB: HeLa and HEK-293T whole cell lysates. IP: HEK-293T whole cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Cdc25A protein (ab90763)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab989于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at 2-10 µg/mg of lysate.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
说明
IP
Use at 2-10 µg/mg of lysate.
WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • 序列相似性

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 结构域

    The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • 翻译后修饰

    Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Target information above from: UniProt accession P30304 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 993 Human
    • Omim: 116947 Human
    • SwissProt: P30304 Human
    • Unigene: 437705 Human
    • 别名

      • Cdc 25a antibody
      • CDC25A antibody
      • CDC25A2 antibody
      • CDC25A2 CAG isoform antibody
      • Cell division cycle 25 homolog A (S. pombe) antibody
      • Cell division cycle 25A antibody
      • Cell division cycle 25A isoform a antibody
      • Cell division cycle 25A isoform b antibody
      • D9Ertd393e antibody
      • Dual specificity phosphatase Cdc25A antibody
      • M phase inducer phosphatase 1 antibody
      • M-phase inducer phosphatase 1 antibody
      • MGC115549 antibody
      • MPIP1_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Cdc25A antibody (ab989)
      Western blot - Anti-Cdc25A antibody (ab989)
      All lanes : Anti-Cdc25A antibody (ab989) at 1 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 59 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-Cdc25A antibody (ab989)
      Immunoprecipitation - Anti-Cdc25A antibody (ab989)

      Cdc25A was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab989 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab989 at 1 µg/ml. 

      Lane 1: ab989 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (11)

    发表研究结果有使用 ab989?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab989 被引用在 11 文献中.

    • Das S  et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
    • Wang Y  et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703
    • Yin W  et al. Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p. Onco Targets Ther 13:3689-3701 (2020). PubMed: 32431519
    • Huang E  et al. CircRNA hsa_circ_0004771 promotes esophageal squamous cell cancer progression via miR-339-5p/CDC25A axis. Epigenomics 12:587-603 (2020). PubMed: 32050790
    • Su J  et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-Cdc25A antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT29 tumour cells)
    Loading amount
    50 µg
    Specification
    HT29 tumour cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Paul Eve

    Verified customer

    提交于 Jan 09 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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