重组Anti-Cdc23抗体[EPR11827] (ab177148)
![Western blot - Anti-Cdc23 antibody [EPR11827] (ab177148)](https://www.abcam.cn/ps/products/177/ab177148/Images/ab177148-205123-ab177148.jpg "Western blot - Anti-Cdc23 antibody [EPR11827] (ab177148)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11827] to Cdc23
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc23抗体[EPR11827]
参阅全部 Cdc23 一抗 -
描述
兔单克隆抗体[EPR11827] to Cdc23 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide within Human Cdc23 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UJX2 -
阳性对照
- HepG2, HeLa, K562 and Jurkat cell lysates. HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR11827 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177148于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 68 kDa).
|
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| ICC/IF |
1/50 - 1/100.
|
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| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 68 kDa). |
|
ICC/IF
1/50 - 1/100. |
|
IP
1/10 - 1/100. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC8/CDC23 family.
Contains 9 TPR repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 8697 Human
- Entrez Gene: 291689 Rat
- Omim: 603462 Human
- SwissProt: Q9UJX2 Human
- Unigene: 73625 Human
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别名
- ANAPC8 antibody
- Anaphase promoting complex subunit 8 antibody
- Anaphase-promoting complex subunit 8 antibody
see all
图片
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Western blot - Anti-Cdc23 antibody [EPR11827] (ab177148)All lanes : Anti-Cdc23 antibody [EPR11827] (ab177148) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11827] (ab177148)](https://www.abcam.cn/ps/products/177/ab177148/Images/ab177148-205124-ab177148b-copy.gif)
Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11827] (ab177148)Immunofluorescent analysis of HeLa cells labeling Cdc23 with ab177148 at 1/50 dilution.
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![Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] (ab177148)](https://www.abcam.cn/ps/products/177/ab177148/Images/ab177148-205125-ab177148c.jpg)
Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] (ab177148)Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-Cdc23 antibody [EPR11827] (ab177148)](https://www.abcam.cn/ps/products/177/ab177148/Images/ab177148-4-benefits-of-recombinant-antibodies.png)
Anti-Cdc23 antibody [EPR11827] (ab177148)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab177148 被引用在 1 文献中.
- Zhang Y et al. CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. Oncol Lett 26:291 (2023). PubMed: 37274472