Anti-Cdc20抗体[BA8] (ab18217)
![Flow Cytometry - Anti-Cdc20 antibody [BA8] (ab18217)](https://www.abcam.cn/ps/products/18/ab18217/Images/ab18217-27524-anti-cdc20-antibody-ba8-flow-cytometry.jpg "Flow Cytometry - Anti-Cdc20 antibody [BA8] (ab18217)")
Key features and details
- Mouse monoclonal [BA8] to Cdc20
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cdc20抗体[BA8]
参阅全部 Cdc20 一抗 -
描述
小鼠单克隆抗体[BA8] to Cdc20 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic C terminal peptide (Xenopus laevis).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
BA8 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
发展阶段
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
翻译后修饰
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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数据库链接
- Entrez Gene: 991 Human
- Omim: 603618 Human
- SwissProt: Q12834 Human
- Unigene: 524947 Human
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别名
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
图片
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Flow Cytometry - Anti-Cdc20 antibody [BA8] (ab18217)Overlay histogram showing LoVo cells stained with ab18217 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18217, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab18217 被引用在 4 文献中.
- Heim A et al. Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20. EMBO Rep 19:N/A (2018). PubMed: 30373936
- Qiao R et al. Mechanism of APC/CCDC20 activation by mitotic phosphorylation. Proc Natl Acad Sci U S A 113:E2570-8 (2016). PubMed: 27114510
- Eytan E et al. Roles of different pools of the mitotic checkpoint complex and the mechanisms of their disassembly. Proc Natl Acad Sci U S A 110:10568-73 (2013). PubMed: 23754430
- Ohe M et al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). WB ; Human, Xenopus laevis . PubMed: 20089832