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AB18217

Anti-Cdc20抗体[BA8]

Anti-Cdc20 antibody [BA8]

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(4 Publications)

Mouse Monoclonal CDC20 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Cell division cycle protein 20 homolog.

查看别名

Cell division cycle protein 20 homolog, p55CDC, CDC20

1 Images
Flow Cytometry - Anti-Cdc20 antibody [BA8] (AB18217)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cdc20 antibody [BA8] (AB18217)

Overlay histogram showing LoVo cells stained with ab18217 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18217, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

BA8

亚型

IgG2a

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt

applications

免疫原

Synthetic Peptide within Human Cell division cycle protein 20 homolog. The exact immunogen used to generate this antibody is proprietary information.

Q12834

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.02% Sodium azide Constituents: 99.98% PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.
Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex (PubMed : 27030811). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).
See full target information Cell division cycle protein 20 homolog
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 19: PubMed30373936

2018

Calcineurin promotes APC/C activation at meiotic exit by acting on both XErp1 and Cdc20.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Heim,Thomas Tischer,Thomas U Mayer

Proceedings of the National Academy of Sciences of 113:E2570-8 PubMed27114510

2016

Mechanism of APC/CCDC20 activation by mitotic phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Renping Qiao,Florian Weissmann,Masaya Yamaguchi,Nicholas G Brown,Ryan VanderLinden,Richard Imre,Marc A Jarvis,Michael R Brunner,Iain F Davidson,Gabriele Litos,David Haselbach,Karl Mechtler,Holger Stark,Brenda A Schulman,Jan-Michael Peters

Proceedings of the National Academy of Sciences of the United States of America 110:10568-73 PubMed23754430

2013

Roles of different pools of the mitotic checkpoint complex and the mechanisms of their disassembly.

Applications

Unspecified application

Species

Unspecified reactive species

Esther Eytan,Danielle Sitry-Shevah,Adar Teichner,Avram Hershko

Molecular biology of the cell 21:905-13 PubMed20089832

2010

Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail.

Applications

WB, WB

Species

Human, Xenopus laevis

Munemichi Ohe,Yoshiko Kawamura,Hiroyuki Ueno,Daigo Inoue,Yoshinori Kanemori,Chiharu Senoo,Michitaka Isoda,Nobushige Nakajo,Noriyuki Sagata
View all publications
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