Anti-Cdc20抗体(ab26483)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdc20抗体
参阅全部 Cdc20 一抗 -
描述
兔多克隆抗体to Cdc20 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Cdc20.
参阅Abcam的专有抗源政策 (Peptide available as ab28138.) -
阳性对照
- This antibody gave a positive result in the following whole cell lysates: Raji (Human Burkitt's lymphoma cell line) THP1 (Human acute monocytic leukemia cell line) HEK 293 (Human embryonic kidney cell line) HL60 (Human promyelocytic leukemia cell line)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
发展阶段
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
翻译后修饰
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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数据库链接
- Entrez Gene: 991 Human
- Entrez Gene: 107995 Mouse
- Entrez Gene: 397379 Pig
- Entrez Gene: 64515 Rat
- Omim: 603618 Human
- SwissProt: Q12834 Human
- SwissProt: Q9JJ66 Mouse
- SwissProt: Q5H7C0 Pig
see all -
别名
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (ab26483)ab26483 staining Cdc20 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab26483 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Cdc20 antibody (ab26483)All lanes : Anti-Cdc20 antibody (ab26483) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)
Lane 2 : THP-1 whole cell lysate (ab7913)
Lane 3 : HEK-293 whole cell lysate (ab7902)
Lane 4 :HL-60 whole cell lysate (ab7914)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab26483 被引用在 17 文献中.
- Zhang N et al. Unique progerin C-terminal peptide ameliorates Hutchinson-Gilford progeria syndrome phenotype by rescuing BUBR1. Nat Aging 3:185-201 (2023). PubMed: 37118121
- Sun C et al. MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma. Onco Targets Ther 13:10475-10487 (2020). PubMed: 33116627
- Movsisyan N & Pardo LA Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel) 12:N/A (2020). PubMed: 32854244
- Huo X et al. Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data. Sci Rep 9:9924 (2019). PubMed: 31289358
- Li J et al. M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s. Cell Div 14:5 (2019). PubMed: 31249607