Anti-Cdc13抗体[6F11/2] (ab10873)
Key features and details
- Mouse monoclonal [6F11/2] to Cdc13
- Suitable for: WB
- Isotype: IgG2a
概述
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产品名称
Anti-Cdc13抗体[6F11/2] -
描述
小鼠单克隆抗体[6F11/2] to Cdc13 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
预测可用于: Schizosaccharomyces pombe -
免疫原
Full length native protein (purified).
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常规说明
Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation. -
克隆
单克隆 -
克隆编号
6F11/2 -
骨髓瘤
Sp2 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10873于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
靶标
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数据库链接
- Entrez Gene: 2540880 Schizosaccharomyces pombe
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别名
- CDC 13 antibody
- G2/mitotic specific cyclin antibody
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab10873 被引用在 7 文献中.
- Basu S et al. Transcription factor Atf1-dependent degradation of the mitotic cyclin Cdc13 is regulated by multiple factors in Schizosaccharomyces pombe. FEBS Lett 596:2021-2030 (2022). PubMed: 35770329
- Basu S et al. The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis. Curr Biol 30:883-892.e4 (2020). PubMed: 32084401
- Rothe C et al. A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. J Cell Sci 130:4028-4037 (2017). PubMed: 29046339
- Uchiyama M et al. The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage. PLoS One 10:e0130000 (2015). PubMed: 26147350
- Bandyopadhyay S et al. The basic leucine zipper domain transcription factor Atf1 directly controls Cdc13 expression and regulates mitotic entry independently of Wee1 and Cdc25 in Schizosaccharomyces pombe. Eukaryot Cell 13:813-21 (2014). Schizosaccharomyces pombe . PubMed: 24728197
- Okuzaki D et al. Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. Cell Cycle 9: (2010). WB ; Schizosaccharomyces pombe . PubMed: 20855961
- Decottignies A et al. In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis. J Cell Sci 114:2627-40 (2001). PubMed: 11683390