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重组RabMAb

重组Anti-CDC123抗体[EP5861] - BSA and Azide free (ab226111)

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Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
  • Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
  • OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
  • Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP5861] to CDC123 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-CDC123抗体[EP5861] - BSA and Azide free
    参阅全部 CDC123 一抗

  • 描述

    兔单克隆抗体[EP5861] to CDC123 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, 293T, Caco 2, RAW264.7 and PC12 cell lysates; HeLa cells.

  • 常规说明

    ab226111 is the carrier-free version of ab129101.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226111于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

图片

  • Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    All lanes : Anti-CDC123 antibody [EP5861] (ab129101) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 39 kDa



    This data was developed using ab129101, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)

    This data was developed using ab129101, the same antibody clone in a different buffer formulation. 

    ab129101, at 1/10 dilution staining CDC123 in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green). 

     

     

  • OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    This data was developed using ab129101, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
    Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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