重组Anti-CDC123抗体[EP5861] (ab129101)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5861] to CDC123
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CDC123抗体[EP5861]
参阅全部 CDC123 一抗 -
描述
兔单克隆抗体[EP5861] to CDC123 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, 293T, Caco 2, RAW264.7 and PC12 cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.15 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP5861 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa). |
靶标
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功能
Required for S phase entry of the cell cycle. -
组织特异性
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis. -
序列相似性
Belongs to the CDC123 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8872 Human
- Entrez Gene: 98828 Mouse
- Entrez Gene: 116656 Rat
- SwissProt: O75794 Human
- SwissProt: Q8CII2 Mouse
- SwissProt: Q62834 Rat
- Unigene: 412842 Human
- Unigene: 181490 Mouse
see all -
别名
- C10orf7 antibody
- CD123_HUMAN antibody
- CDC123 antibody
see all
图片
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All lanes : Anti-CDC123 antibody [EP5861] (ab129101) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
ab129101, at 1/10 dilution staining CDC123 in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab129101 尚未被引用在任何文献中。