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Immunology Cell Type Markers CD Non-lineage

Anti-CD98抗体[MEM-108] - Plasma膜Marker (ab2528)

  • Datasheet
  • SDS
Reviews (3)Q&A (5)References (2)

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Flow Cytometry - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)

    Key features and details

    • Mouse monoclonal [MEM-108] to CD98 - Plasma Membrane Marker
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

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    概述

    • 产品名称

      Anti-CD98抗体[MEM-108] - Plasma膜Marker
      参阅全部 CD98 一抗
    • 描述

      小鼠单克隆抗体[MEM-108] to CD98 - Plasma膜Marker
    • 宿主

      Mouse
    • 经测试应用

      适用于: Flow Cytmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Tissue, cells or virus corresponding to Human CD98. Burkitt’s lymphoma cell line Raji

    • 阳性对照

      • Flow Cyt: Human peripheral blood monocytes.
    • 常规说明

      CD98 (4F2) is involved in normal and neoplastic cell growth.

      This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.
    • Primary antibody说明

      CD98 (4F2) is involved in normal and neoplastic cell growth.
    • 克隆

      单克隆
    • 克隆编号

      MEM-108
    • 同种型

      IgG1
    • 研究领域

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calcium Channels
      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Alternative Versions

      • Biotin Anti-CD98 antibody [MEM-108] (ab26009)
      • FITC Anti-CD98 antibody [MEM-108] (ab26010)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human CD98 protein (His tag) (ab181902)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2528于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use a concentration of 4 µg/ml.
    说明
    Flow Cyt
    Use a concentration of 4 µg/ml.

    靶标

    • 功能

      Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.
    • 组织特异性

      Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
    • 序列相似性

      Belongs to the SLC3A transporter family.
    • 翻译后修饰

      Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
    • 细胞定位

      Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
    • Target information above from: UniProt accession P08195 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 6520 Human
      • Omim: 158070 Human
      • SwissProt: P08195 Human
      • Unigene: 502769 Human
      • 形式

        There are 4 isoforms produced by alternative splicing.
      • 别名

        • 4F2 antibody
        • 4F2 cell surface antigen heavy chain antibody
        • 4F2 cell-surface antigen heavy chain antibody
        • 4F2 heavy chain antibody
        • 4F2 heavy chain antigen antibody
        • 4F2_HUMAN antibody
        • 4F2hc antibody
        • 4T2HC antibody
        • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
        • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
        • CD 98 antibody
        • CD98 antibody
        • CD98 antigen antibody
        • CD98 heavy chain antibody
        • CD98HC antibody
        • Heavy chain antibody
        • Lymphocyte activation antigen 4F2 large subunit antibody
        • MDU 1 antibody
        • MDU1 antibody
        • Monoclonal antibody 44D7 antibody
        • NACAE antibody
        • Slc3a2 antibody
        • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
        • Solute carrier family 3 member 2 antibody
        see all

      图片

      • Flow Cytometry - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
        Flow Cytometry - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)

        Flow Cytometry analysis of human peripheral blood monocytes labelling CD98 with ab2528 at 4 μg/ml.

      实验方案

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab2528?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2528 被引用在 2 文献中.

      • Millen S  et al. Collagen IV (COL4A1, COL4A2), a Component of the Viral Biofilm, Is Induced by the HTLV-1 Oncoprotein Tax and Impacts Virus Transmission. Front Microbiol 10:2439 (2019). PubMed: 31708905
      • Gong S  et al. Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells. SLAS Discov 23:832-841 (2018). PubMed: 29505735

      客户评价及客户问答

      Show All 评价 Q&A
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      1-8 of 8 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanoma cell line)
      Permeabilization
      No
      Specification
      Melanoma cell line
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 17 2021

      Western blot abreview for Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Melanoma cell line)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      40 µg
      Specification
      Melanoma cell line
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 16 2021

      Immunoprecipitation abreview for Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (Melanoma cell line)
      Total protein in input
      1000 µg
      Immuno-precipitation step
      Protein A/G
      Specification
      Melanoma cell line
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Praveen Agrawal

      Verified customer

      提交于 Jun 16 2021

      Question

      Thank you for your email. We've now rerun the samples and unfortunately it's still not very good.
      We know the cells are definitely positive for CD98 because we've done extracellular flow cytometry on them and they stain nicely (have very high levels). Our lysis protocol works nicely because we see good staining of mouse tumour cells with a different CD98 antibody.

      Please find attached a picture of 2 westerns done using your suggested modifications.
      The first was detecting using a pico-HRP substrate (all we are detecting is marker) and the second is using a much more sensitive femto reagent. We know the proteins transferred well to the membrane, there is just very little sensitivity and a lot of background (the third lane in each occasion is molecular weight markers. The femto reagent had to be exposed for a long time as you can see from the marker lane.

      Would it be possible to get a refund on this product please as we've now bought a replacement antibody from a different company?
      Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2012

      Answer

      Thank you for your email.
      I am sorry to hear that the product failed again. I have now raised the quality issues to our qc team. They might decide to retest this product.
      I appreciate the time you have spent in troubleshooting and for the cooperation while dealing with this case. It is very unfortunate that the product did not give expected results.
      I have put forward a request to our accounting department for refund. The refund ID is 23079. The refund note may be used in one of the following ways:
      (1) Redeemed against the original invoice if this hasn't already been paid.
      (2) Held on the account for use against a future order.
      (3) A full refund can be offered where no other invoices are outstanding.
      Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
      To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
      The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
      I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

      Read More

      Abcam Scientific Support

      回复于 Oct 17 2012

      Question

      Please find attached the questionnaire regarding our recent antibody experiences. I've also attached a jpg of the CD98 and control western blot stains.
      It would be good to get an alternative antibody as soon as possible as we need to get this project moving forward.
      Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 09 2012

      Answer

      Thank you for contacting us. I apologies for the delay.
      The protocol seems fine to me however I would suggest following updates;
      - Blocking time; I can recommend blocking the membrane for 1-2 hours in 5% BSA at RT, I recommended BSA because it works better with some antibodies which is why we commonly use it in our testing lab.
      - Heat the protein in sample buffer for 10 minutes at 100C.
      Please note as CD98 is a heavily glycosylated protein so the appearance of band will be like smear rather than sharp one.
      I am sure these simple changes will help to improve results. Please do not hesitate to contact me if antibody fails again.
      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Oct 09 2012

      Question

      - Antibody fails in WB
      - Multiple bands around band of interest

      Read More

      Abcam community

      Verified customer

      Asked on Oct 05 2012

      Answer

      Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
      As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      回复于 Oct 05 2012

      Question

      Inquiry: The CD98 protein is a heterodimer, composed of a heavy chain and a light chain linked by disulfide bonds. We're looking for an antibody staining ONLY the HEAVY CHAIN of CD98 (also named CD98hc, 4F2hc or SLC3A2) by immunofluorescence. The antibody clone MEM-108 is described as recognizing CD98, but it is not clear whether it would stains one or the other (or both) of the chains. The western-blot shown in the datasheet does not give a clear answer because the high molecular weigth signal suggests that the western-blot was done on a native gel (the expected size of the heterodimer is 125kDa whereas the heavy chain is approx. 80kDa and the light-chain 40kDa). Even if the heavy chain is highly glycosylated, we should expect a smaller band than what is shown on the figure... Do you have more info about the specificity of this antibody or about how this western-blot was performed ? Many thanks in advance for your help.

      Read More

      Abcam community

      Verified customer

      Asked on May 31 2012

      Answer

      Thank you for contacting us and sorry for the delay of our reply.

      Please find below the information I obtained for each anti-CD98 antibodies:

      ab2528 : the epitope hasnot beenmapped.
      ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
      ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



      I would recommend the antibody https://www.abcam.com/ab108300.
      To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

      2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

      3. Test it in ICC/IF.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      回复于 May 31 2012

      Question

      Dear Sir/Madame, do you have any info on the reactivity of anti CD98 antibody with rat cells?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 21 2004

      Answer

      Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

      Read More

      Abcam Scientific Support

      回复于 Apr 21 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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