重组Anti-CD97抗体[EPR4428] (ab134105)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4428] to CD97
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CD97抗体[EPR4428]
参阅全部 CD97 一抗 -
描述
兔单克隆抗体[EPR4428] to CD97 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: U937 and THP-1 cell lysates IHC-P: Human colonic adenocarcinoma tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4428 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134105于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
|
|
IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa. |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. -
组织特异性
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain. -
结构域
The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain. -
翻译后修饰
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
-
数据库链接
- Entrez Gene: 976 Human
- Entrez Gene: 361383 Rat
- Omim: 601211 Human
- SwissProt: P48960 Human
- Unigene: 466039 Human
-
别名
- ADGRE5 antibody
- Adhesion G protein-coupled receptor E5 antibody
- CD 97 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] (ab134105)
Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Lane 1 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/10000 dilution
Lane 2 : Anti-CD97 antibody [EPR4428] (ab134105) at 1/20000 dilution
Lane 1 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration 5% NFDM/TBST
Exposure time:
Lane 1: 180 seconds
Lane 2: 60 secondsThe 28kDa is β-chain. This is supported by many papers, PMID: 20084281, PMID: 18941248.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab134105 尚未被引用在任何文献中。