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Immunology Adaptive Immunity T Cells CD

Anti-CD90 / Thy1抗体[MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

  • Datasheet
  • SDS
Reviews (12)Q&A (5)References (97)

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Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

Key features and details

  • Mouse monoclonal [MRC OX-7] to CD90 / Thy1 - Hematopoietic Stem Cell Marker
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Rat
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC HRP PE PerCP

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概述

  • 产品名称

    Anti-CD90 / Thy1抗体[MRC OX-7] - Hematopoietic Stem Cell Marker
    参阅全部 CD90 / Thy1 一抗
  • 描述

    小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 - Hematopoietic Stem Cell Marker
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC, WB, Flow Cyt (Intra)more details
  • 种属反应性

    与反应: Rat
    预测可用于: Mouse, Rabbit, Horse, Guinea pig
  • 免疫原

    Full length protein corresponding to Rat CD90/ Thy1.

  • 阳性对照

    • WB: Rat brain tissue lysate and PC12 whole cell lysate. ICC: PC12 cells. Flow Cyt (Intra): PC12 cells.
  • 常规说明

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    MRC OX-7
  • 骨髓瘤

    NS1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

相关产品

  • Alternative Versions

    • HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
    • PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
    • Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
    • FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
    • PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
Use a concentration of 1 µg/ml.
WB (2)
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
Flow Cyt (Intra)
Use 0.1µg for 101-6 cells.
说明
ICC
Use a concentration of 1 µg/ml.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
Flow Cyt (Intra)
Use 0.1µg for 101-6 cells.

靶标

  • 功能

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P04216 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 21838 Mouse
    • Entrez Gene: 24832 Rat
    • SwissProt: P01831 Mouse
    • SwissProt: P01830 Rat
    • Unigene: 3951 Mouse
    • Unigene: 108198 Rat
    • 别名

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

      Overlay histogram showing PC12 cells stained with ab225 (red line).

      The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC.

      The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ab96879 at 1/500 dilution for 30 min at 22ºC.

      Isotype control antibody (black line, mouse IgG1 [B11/6], ab91353, 1µg/1x106 cells) used under the same conditions.

      Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

      Ab225 gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

      ab225 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab225 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)This image is courtesy of an abreview submitted by Vega Villar, University of Leon.

      Immunocytochemistry/ Immunofluorescence analysis of horse adipose and bone marrow stem cells labeling CD90 / Thy1 with ab225 at 1/1000 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton x100 0.1%. The cells were blocked with 10% BSA for 30 minutes at 37°C, followed by staining with ab225 at 1/1000 for 12 hours in PBS+IGEPAL+BSA+10%NGS at 4°C. Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886) was used as the secondary antibody at 1/400 dilution.

      See Abreview

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)This image is courtesy of an anonymous abreview.

      Immunocytochemistry/ Immunofluorescence analysis of rat sciatic nerve schwann cells and fibroblasts labeling CD90 / Thy1 with ab225 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100. The cells were blocked with 5% serum for 1 hour at 21°C, followed by staining with ab225 at 1/400 in 0.2% BSA for 1 hour at 37°C. A polyclonal goat anti-mouse Alexa Fluor® 546 secondary antibody was used at 1/500 dilution.

      See Abreview

    • Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225) at 5 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate
      Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 17 kDa
      Observed band size: 35-37 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      Rat CD90/Thy1 is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25-37 kDa). 

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      This image was kindly supplied as part of the review submitted by Nick Voilley. Rat retinal ganglion cell labelled with ab225 as a primary antibody and an anti-mouse F(ab)' 2 coupled to Alexa488 as a secondary antibody. The cell is approximately 15 micrometers in diameter. Only the cells labelled in green in the culture bear action potentials when stimulated. These three elements (reactivity to ab225, size and elecrophysiological parameters) clearly indicate the cell is a ganglion cell.

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (97)

    发表研究结果有使用 ab225?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab225 被引用在 97 文献中.

    • Wu H  et al. miR-34a in extracellular vesicles from bone marrow mesenchymal stem cells reduces rheumatoid arthritis inflammation via the cyclin I/ATM/ATR/p53 axis. J Cell Mol Med 25:1896-1910 (2021). PubMed: 33465281
    • Liang L  et al. Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther 12:87 (2021). PubMed: 33494812
    • Liang Q  et al. Stromal cell-derived factor-1/Exendin-4 cotherapy facilitates the proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells in vitro and promotes periodontal bone regeneration in vivo. Cell Prolif 54:e12997 (2021). PubMed: 33511708
    • Bourebaba L  et al. MSI-1436 improves EMS adipose derived progenitor stem cells in the course of adipogenic differentiation through modulation of ER stress, apoptosis, and oxidative stress. Stem Cell Res Ther 12:97 (2021). PubMed: 33536069
    • Zhang J  et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cow Cell (MSCs bovine cells)
    Permeabilization
    No
    Gating Strategy
    All cells
    Specification
    MSCs bovine cells
    Preparation
    Cell harvesting/tissue preparation method: 100,000 cells were placed in each well (96 wells) in a volume of 100ul and the first antibody was added, after incubation and washes the secondary antibody was added and incubated (in the dark) following washes. Afterward, the sample was read by the flow cytometer
    Sample buffer: 3% BSA in PBS
    Fixation
    The cells weren't fixated
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 30 2022

    Western blot abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BRAIN)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    30 µg
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 27 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Primary bone marrow-derived stromal cells)
    Permeabilization
    No
    Specification
    Primary bone marrow-derived stromal cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Egi Kardia

    Verified customer

    提交于 Sep 21 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Bone and cartilage)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    Bone and cartilage
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    70 % Ethanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 26 2014

    Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (mMSC)
    Permeabilization
    No
    Specification
    mMSC
    Preparation
    Cell harvesting/tissue preparation method: bone marrow extraction
    Sample buffer: PBS
    Fixation
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 19 2012

    Western blot abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mMSC)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    70 µg
    Specification
    mMSC
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 18 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    FBS / BSA in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Raul Peña

    Verified customer

    提交于 Dec 03 2012

    Question

    Do cells need to be permeabilized before using this antibody in flow cytometry?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you very much for your call yesterday and for your patience.
    I have confirmed with the lab that the antibody is tested with non-permeabilized cells in flow cytometry. So, the epitope is extracellular.
    I hope that this information will be useful, but if you have any further questions please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    回复于 Aug 08 2012

    Question

    Dear technical support team:
    This customer has highly interested in ab225 (Anti-CD90 / Thy1 antibody [MRC OX-7]), he would likes to order this antibody and test in IHC-Fr with porcine sample, would you please help this customer to create a discount code, the information of this customer as follow:

    Thanks for your kindly help.
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2012

    Answer

    Thank you for your enquiry and your interest.

    Unfortunately, we currently do not have any data (either empirical or from literature) that would support reactivity to porcine tissue. A quick BLAST comparison shows only a 75% homology between the rodent and porcine CD90.

    Based on this,we would expect only a weak reactivity to porcine tissue.

    Read More

    Abcam Scientific Support

    回复于 May 17 2012

    Question

    flow cytometry with human and rabbit mesenchymal stem cells
    problem: no signal
    fix: commercial solution
    ab11414: Ab dilution not sure
    ab225: 1/50
    no blocking step
    2nd: PE-conj., works well
    used controls - also no signal

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab11414andab225(different lots). The order will ship on Monday for delivery on Tuesday.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 May 11 2012

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    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
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