Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证重组RabMAb

重组Anti-CD90 / Thy1抗体[EPR3133] - BSA and Azide free (ab226123)

Reviews (1) Submit a question References (2)
Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
  • Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3133] to CD90 / Thy1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-CD90 / Thy1抗体[EPR3133] - BSA and Azide free
    参阅全部 CD90 / Thy1 一抗

  • 描述

    兔单克隆抗体[EPR3133] to CD90 / Thy1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human fetal brain and Human bone marrow lysates. Human glioma and kidney tissues

  • 常规说明

    ab226123 is the carrier-free version of ab133350.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226123于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.

Observed molecular weight may vary depending on the glycosylation level of the target.
说明
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.

Observed molecular weight may vary depending on the glycosylation level of the target.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    图片

    • Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/2000 dilution

      Lane 1 : Wild-type U-2 OS cell lysate
      Lane 2 : THY1 knockout U-2 OS cell lysate
      Lane 3 : Human brain cell lysate
      Lane 4 : Human kidney cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 17 kDa
      Observed band size: 25-37 kDa why is the actual band size different from the predicted?



      This data was developed using ab133350, the same antibody clone in a different buffer formulation.

      Anti-THY1 antibody [EPR3133] (ab133350) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133350 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      Immunohistochemical analysis of paraffin-embedded Human normal kidney tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      ab133350 staining CD90 / Thy1  in the SH-SY5Y human neuroblastoma epithelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/100). ab150077 an Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI was used as a nuclear counter stain and Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain microtubules.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labelling CD90 / Thy1 with ab133350 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab133350 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      Immunohistochemical analysis of paraffin embedded normal Human tonsil vessels tissue using ab133350 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

      Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab133350 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133350).

    • Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
      Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (2)

    发表研究结果有使用 ab226123？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226123 被引用在 2 文献中.

    • Yuan X  et al. Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying a bispecific T cell engager against hepatocellular carcinoma. Oncoimmunology 12:2219544 (2023). PubMed: 37274296
    • Lin B  et al. Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation. Cell Death Discov 8:160 (2022). PubMed: 35379779

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: tris-EDTA ph9
    Permeabilization
    Yes - tween
    Specification
    Lung cancer
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 23 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com