重组Anti-CD90 / Thy1抗体[EPR3133] (ab133350)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3133] to CD90 / Thy1
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD90 / Thy1抗体[EPR3133]
参阅全部 CD90 / Thy1 一抗 -
描述
兔单克隆抗体[EPR3133] to CD90 / Thy1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC: Human tonsil tissue; WB: Human glioma and brain tissue lysate; ICC/IF: Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3133 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 568 Anti-CD90 / Thy1 antibody [EPR3133] (ab201848)
- Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3133] (ab202334)
- Alexa Fluor® 555 Anti-CD90 / Thy1 antibody [EPR3133] (ab202511)
- Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3133] (ab202512)
- Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)
- Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
Observed molecular weight may vary depending on the glycosylation level of the target. |
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ICC/IF | (1) |
1/50.
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说明 |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. Observed molecular weight may vary depending on the glycosylation level of the target. |
ICC/IF
1/50. |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7070 Human
- Omim: 188230 Human
- SwissProt: P04216 Human
- Unigene: 644697 Human
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/2000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : Human kidney cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25-37 kDa why is the actual band size different from the predicted?Anti-THY1 antibody [EPR3133] (ab133350) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133350 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution (Purified)
Lane 1 : Human glioma lysate
Lane 2 : Human brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 25-35 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 24116172 and PMID: 30177788).
Blocking Buffer and concentration: 5% NFDM/TBST
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Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD90 / Thy1 with Purified ab133350 at 1:50 dilution (2.44 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD90 / Thy1 with Purified ab133350 at 1:4000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (69)
ab133350 被引用在 69 文献中.
- Ivanov AA et al. Decellularized Matrix Induced Spontaneous Odontogenic and Osteogenic Differentiation in Periodontal Cells. Biomolecules 13:N/A (2023). PubMed: 36671506
- Chen L et al. THY1 (CD90) Maintains the Adherens Junctions in Nasopharyngeal Carcinoma via Inhibition of SRC Activation. Cancers (Basel) 15:N/A (2023). PubMed: 37046850
- Park C et al. Self-assembled adipose-derived mesenchymal stem cells as an extracellular matrix component- and growth factor-enriched filler. Front Cell Dev Biol 11:1219739 (2023). PubMed: 37799276
- Mikkelsen E et al. mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes? Int J Mol Sci 24:N/A (2023). PubMed: 37958809
- Chen TC & Chang SW Repeated cell sorting ensures the homogeneity of ocular cell populations expressing a transgenic protein. PLoS One 17:e0265183 (2022). PubMed: 35333876