Anti-CD9抗体[TS9] - BSA and Azide free (ab58989)
![Western blot - Anti-CD9 antibody [TS9] (ab58989)](https://www.abcam.cn/ps/products/58/ab58989/Images/ab58989-458923-anti-cd9-antibody-ts9-western-blot-wildtype-hela-no-dtt-cd9-knockout-hela-no-dtt-a549-nonreducing-mcf7-non.jpg "Western blot - Anti-CD9 antibody [TS9] (ab58989)")
Key features and details
- Mouse monoclonal [TS9] to CD9 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD9抗体[TS9] - BSA and Azide free
参阅全部 CD9 一抗 -
描述
小鼠单克隆抗体[TS9] to CD9 - BSA and Azide free -
宿主
Mouse -
特异性
Recognises the p24 antigen, a 24 kda protein.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD9. HeLa cell line
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阳性对照
- WB: HeLa, A549, MCF7 and HEL cell lysates.
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常规说明
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
无载体
是 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
TS9 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58989于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa. |
靶标
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功能
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
组织特异性
Expressed by a variety of hematopoietic and epithelial cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
翻译后修饰
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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别名
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
图片
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Western blot - Anti-CD9 antibody [TS9] (ab58989)All lanes : Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) at 1 µg/ml
Lane 1 : Wild-type HeLa (No DTT) cell lysate
Lane 2 : CD9 knockout HeLa (No DTT) cell lysate
Lane 3 : A549 (Non-Reducing) cell lysate
Lane 4 : MCF7 (Non-Reducing) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab58989 observed at 24 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab58989 was shown to react with CD9 in wild-type HeLa cells in Western blot with loss of signal observed in CD9 knockout cell line ab255375 (CD9 knockout cell lysate ab263754). Wild-type HeLa and CD9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58989 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-CD9 antibody [TS9] (ab58989)](https://www.abcam.cn/ps/products/58/ab58989/Images/ab58989-314974-anti-cd9-antibody-ts9-western-blot.jpg)
Western blot - Anti-CD9 antibody [TS9] (ab58989)Anti-CD9 antibody [TS9] - BSA and Azide free (ab58989) + HEL cell lysate at 130000 cells
Performed under non-reducing conditions.
Predicted band size: 24 kDaThis image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (11)
ab58989 被引用在 11 文献中.
- Ter-Ovanesyan D et al. Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins. Elife 12:N/A (2023). PubMed: 37252755
- Li X et al. Single-cell trajectory analysis reveals a CD9 positive state to contribute to exit from stem cell-like and embryonic diapause states and transit to drug-resistant states. Cell Death Discov 9:285 (2023). PubMed: 37542044
- Couto-Rodriguez A et al. Platelet-Derived Extracellular Vesicles as Lipid Carriers in Severe Allergic Inflammation. Int J Mol Sci 24:N/A (2023). PubMed: 37628895
- Kim HJ et al. Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles. Commun Biol 6:885 (2023). PubMed: 37644220
- Zhou W et al. Engineered exosomes loaded with miR-449a selectively inhibit the growth of homologous non-small cell lung cancer. Cancer Cell Int 21:485 (2021). PubMed: 34521413
