Anti-CD86抗体[GL-1] (ab119857)
Key features and details
- Rat monoclonal [GL-1] to CD86
- Suitable for: Blocking, IP, ICC/IF, Functional Studies, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
概述
-
产品名称
Anti-CD86抗体[GL-1]
参阅全部 CD86 一抗 -
描述
大鼠单克隆抗体[GL-1] to CD86 -
宿主
Rat -
经测试应用
适用于: Blocking, IP, ICC/IF, Functional Studies, Flow Cytmore details
不适用于: IHC-Fr -
种属反应性
与反应: Mouse -
免疫原
LPS-activated CBA/Cs Mouse splenic B cells.
-
阳性对照
- Mouse splenocytes.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. -
克隆
单克隆 -
克隆编号
GL-1 -
同种型
IgG2a -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119857于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Blocking |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Functional Studies |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Blocking
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Functional Studies
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
组织特异性
Expressed by activated B-lymphocytes and monocytes. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 12524 Mouse
- SwissProt: P42082 Mouse
- Unigene: 1452 Mouse
-
别名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (35)
ab119857 被引用在 35 文献中.
- Lv Y et al. Low ferroptosis score predicts chemotherapy responsiveness and immune-activation in colorectal cancer. Cancer Med 12:2033-2045 (2023). PubMed: 35855531
- Ma X et al. Gamma-Aminobutyric Acid Promotes Beige Adipocyte Reconstruction by Modulating the Gut Microbiota in Obese Mice. Nutrients 15:N/A (2023). PubMed: 36678326
- Liu J et al. Medicinal plant-derived mtDNA via nanovesicles induces the cGAS-STING pathway to remold tumor-associated macrophages for tumor regression. J Nanobiotechnology 21:78 (2023). PubMed: 36879291
- Marquez-Ortiz RA et al. Neuroimmune Support of Neuronal Regeneration and Neuroplasticity following Cerebral Ischemia in Juvenile Mice. Brain Sci 13:N/A (2023). PubMed: 37759938
- Zhang Y et al. A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration. Int J Mol Sci 24:N/A (2023). PubMed: 37762541