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重组RabMAb

重组Anti-CD84抗体[EPR8324] - BSA and Azide free (ab226163)

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Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
  • Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
  • Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8324] to CD84 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

概述

  • 产品名称

    Anti-CD84抗体[EPR8324] - BSA and Azide free
    参阅全部 CD84 一抗

  • 描述

    兔单克隆抗体[EPR8324] to CD84 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cytmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Namalwa and Raji cell lysates; Raji cells

  • 常规说明

    ab226163 is the carrier-free version of ab137049.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226163于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).
Flow Cyt
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.

  • 组织特异性

    Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.

  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

  • 发展阶段

    Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.

  • 结构域

    ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.

  • 翻译后修饰

    Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
    N-glycosylated.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD_antigen=CD84 antibody
      • CD84 antibody
      • CD84 antigen antibody
      • CD84 molecule antibody
      • CDw84 antibody
      • Cell surface antigen MAX.3 antibody
      • hCD84 antibody
      • Hly9 beta antibody
      • Hly9-beta antibody
      • Leucocyte differentiation antigen CD84 antibody
      • Leukocyte Antigen antibody
      • Leukocyte antigen CD84 antibody
      • Leukocyte differentiation antigen antibody
      • Leukocyte differentiation antigen CD84 antibody
      • LY9B antibody
      • mCD84 antibody
      • Signaling lymphocytic activation molecule 5 antibody
      • SLAF5_HUMAN antibody
      • SLAM family member 5 antibody
      • SLAMF5 antibody
      see all

    图片

    • Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
      Western blot - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
      All lanes : Anti-CD84 antibody [EPR8324] (ab137049) at 1/1000 dilution

      Lane 1 : Namalwa cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 39 kDa



      This data was developed using ab137049, the same antibody clone in a different buffer formulation.

    • Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
      Flow Cytometry - Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
      This data was developed using ab137049, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Raji cells labelling CD84 with ab137049 at dilution 1/100 (red) or Rabbit IgG negative control (green).
    • Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)
      Anti-CD84 antibody [EPR8324] - BSA and Azide free (ab226163)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226163？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226163 尚未被引用在任何文献中。

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