重组Anti-CD83抗体[HB15e] (ab123494)
![Flow Cytometry - Anti-CD83 antibody [HB15e] (ab123494)](https://www.abcam.cn/ps/products/123/ab123494/Images/ab123494-496158-antiad83antibodyhb15eflowcytgmcsftreatedpbmchuman.jpg "Flow Cytometry - Anti-CD83 antibody [HB15e] (ab123494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [HB15e] to CD83
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD83抗体[HB15e]
参阅全部 CD83 一抗 -
描述
小鼠单克隆抗体[HB15e] to CD83 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details
不适用于: ICC/IF,IHC-P or WB -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: GM-CSF IL-4 treated human peripheral blood mononuclear cells. LPS treated human monocyte-derived dendritic cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HB15e -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/400.
|
| 说明 |
|---|
|
Flow Cyt
1/400. |
靶标
-
功能
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. -
组织特异性
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9308 Human
- Omim: 604534 Human
- SwissProt: Q01151 Human
- Unigene: 595133 Human
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别名
- B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
- B cell activation protein antibody
- B-cell activation protein antibody
see all
图片
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Flow Cytometry - Anti-CD83 antibody [HB15e] (ab123494)
Flow Cytometry analysis of human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days then change new medium (100ng/ml GM-CSF and 50ng/ml IL-4) for another 3 days (right panel) compared to mouse monoclonal IgG isotype control (left panel).
AB123494 used at a 1/400 dilution.
Secondary is Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) used at a 1/2000 dilution.
Gated on viable cells.
Bright secondary antibody like Alexa Fluor®647 conjugated ones are recommended to get desirable signal. -
![Flow Cytometry - Anti-CD83 antibody [HB15e] (ab123494)](https://www.abcam.cn/ps/products/123/ab123494/Images/ab123494-496159-antiad83antibodyhb15eflowcytlpstreatedmonocytederiveddendritichuman.jpg)
Flow Cytometry - Anti-CD83 antibody [HB15e] (ab123494)Flow Cytometry analysis of human monocyte-derived dendritic cells treated with 100ng/mL Lipopolysaccharide (LPS) for 24 hours (Right) / Untreated control (Left).
AB123494 used at a 1/1000 dilution.
Secondary is Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) used at a 1/2000 dilution.
The cells were simultaneously stained with CD209. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
Events were gated on viable single cells. -
![Anti-CD83 antibody [HB15e] (ab123494)](https://www.abcam.cn/ps/products/123/ab123494/Images/ab123494-496309-BenefitsofRecombinantantibodies.jpg)
Anti-CD83 antibody [HB15e] (ab123494)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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