Anti-CD82抗体[B-L2] - BSA and Azide free (ab47153)
Key features and details
- Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD82抗体[B-L2] - BSA and Azide free
参阅全部 CD82 一抗 -
描述
小鼠单克隆抗体[B-L2] to CD82 - BSA and Azide free -
宿主
Mouse -
特异性
Recognises the CD82 antigen, a 60 kDa protein. Cross reacts with mouse CD82 protein in cytometry.
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经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD82. CD82 transfected P815 cell line
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
无载体
是 -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free. -
克隆
单克隆 -
克隆编号
B-L2 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47153于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. -
组织特异性
Lymphoid specific. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3732 Human
- Omim: 600623 Human
- SwissProt: P27701 Human
- Unigene: 527778 Human
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别名
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
图片
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A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] (ab47153)IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab47153 被引用在 3 文献中.
- Di Giacomo V et al. ?Np63a promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82. Oncogene 36:4381-4392 (2017). Flow Cyt . PubMed: 28368419
- Marjon KD et al. Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin. Oncogene 35:4132-40 (2016). ICC/IF . PubMed: 26592446
- Artavanis-Tsakonas K et al. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. Infect Immun : (2010). ICC/IF ; Human . PubMed: 21149584