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重组RabMAb

重组Anti-CD80抗体[EPR22183] (ab225674)

Reviews (1) Submit a question References (3)
Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
  • Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)
  • Anti-CD80 antibody [EPR22183] (ab225674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22183] to CD80
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

概述

  • 产品名称

    Anti-CD80抗体[EPR22183]
    参阅全部 CD80 一抗

  • 描述

    兔单克隆抗体[EPR22183] to CD80

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, Flow Cyt, IPmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: Raji cells. IP: Raji whole cell lysate. Flow cyt: Raji cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225674于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
1/1000.
Flow Cyt
1/400.
IP
1/30.
说明
ICC/IF
1/1000.
Flow Cyt
1/400.
IP
1/30.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

  • 组织特异性

    Expressed on activated B-cells, macrophages and dendritic cells.

  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Activation B7-1 antigen antibody
      • B lymphocyte activation antigen B7 antibody
      • B7 antibody
      • B7-1 antibody
      • B7-1 antigen antibody
      • B7.1 antibody
      • BB1 antibody
      • CD28 antigen ligand 1 antibody
      • CD28LG antibody
      • CD28LG1 antibody
      • CD80 antibody
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
      • CD80 antigen antibody
      • CD80 molecule antibody
      • CD80_HUMAN antibody
      • Costimulatory factor CD80 antibody
      • costimulatory molecule variant IgV-CD80 antibody
      • CTLA-4 counter-receptor B7.1 antibody
      • LAB7 antibody
      • T-lymphocyte activation antigen CD80 antibody
      see all

    图片

    • Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
      Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)

      Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80  with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: HUVEC (PMID: 15064090).

    • Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)

      CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate 10 μg (Input).
      Lane 2: ab225674 IP in Raji whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 second.

      This blot was developed using a higher sensitivity ECL substrate.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution dilution.

      Negative control: HUVEC (PMID: 15064090).

    • Anti-CD80 antibody [EPR22183] (ab225674)
      Anti-CD80 antibody [EPR22183] (ab225674)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (3)

    发表研究结果有使用 ab225674？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab225674 被引用在 3 文献中.

    • Liu B  et al. Dental pulp stem cells induce anti-inflammatory phenotypic transformation of macrophages to enhance osteogenic potential via IL-6/GP130/STAT3 signaling. Ann Transl Med 11:90 (2023). PubMed: 36819570
    • Lee JH  et al. Emulating Early Atherosclerosis in a Vascular Microphysiological System Using Branched Tissue-Engineered Blood Vessels. Adv Biol (Weinh) 5:e2000428 (2021). PubMed: 33852179
    • Ma C  et al. Pterostilbene Alleviates Cholestasis by Promoting SIRT1 Activity in Hepatocytes and Macrophages. Front Pharmacol 12:785403 (2021). PubMed: 34899349

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD80 antibody [EPR22183]

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U937 human histiocytic lymphoma- differentiated ma)
    Permeabilization
    No
    Specification
    U937 human histiocytic lymphoma- differentiated ma
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Esra Sengul

    Verified customer

    提交于 Apr 18 2022

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