Anti-CD80抗体(ab64116)
Key features and details
- Rabbit polyclonal to CD80
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-CD80抗体
参阅全部 CD80 一抗 -
描述
兔多克隆抗体to CD80 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CD80.
参阅Abcam的专有抗源政策 (Peptide available as ab66476.) -
阳性对照
- This antibody gave a positive signal in the following lysates: Mouse Lymph node
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64116于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
组织特异性
Expressed on activated B-cells, macrophages and dendritic cells. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12519 Mouse
- SwissProt: Q00609 Mouse
- Unigene: 89474 Mouse
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别名
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody (ab64116)
ab64116 (1:40) staining CD80 in paraffin-embedded mouse lymph node tissue (left panel) using an automated system (Ventana Discovery). Right-hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining, of activated B cells in the germinal centres. What appear to be scattered activated B cells or possibly plasma cells in the interfollicular areas of the lymph node and blood vessels.Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Extended Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab64116 (1:40 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab64116 被引用在 9 文献中.
- Ceci L et al. Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model. Cell Mol Gastroenterol Hepatol 14:877-904 (2022). PubMed: 35863741
- Annangi B et al. Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis. Part Fibre Toxicol 18:6 (2021). PubMed: 33526046
- Rahmati M et al. Early osteoimmunomodulatory effects of magnesium-calcium-zinc alloys. J Tissue Eng 12:20417314211047100 (2021). PubMed: 34589198
- Tcvetkov N et al. Immune checkpoints bone marrow expression as the predictor of clinical outcome in myelodysplastic syndrome. Leuk Res Rep 14:100215 (2020). PubMed: 32695574
- Zhou X et al. YAP Aggravates Inflammatory Bowel Disease by Regulating M1/M2 Macrophage Polarization and Gut Microbial Homeostasis. Cell Rep 27:1176-1189.e5 (2019). PubMed: 31018132
- Miao Y et al. Adaptive Immune Resistance Emerges from Tumor-Initiating Stem Cells. Cell 177:1172-1186.e14 (2019). PubMed: 31031009
- Peng W et al. Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development. Mol Cell Biol 38:N/A (2018). PubMed: 29133460
- Xu S et al. miR-424(322) reverses chemoresistance via T-cell immune response activation by blocking the PD-L1 immune checkpoint. Nat Commun 7:11406 (2016). WB . PubMed: 27147225
- Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). PubMed: 25845433