Anti-CD8 alpha抗体[YTS169.4] (ab22378)
![Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)](https://www.abcam.cn/ps/products/22/ab22378/Images/ab22378-31688-anti-cd8-antibody-yts1694-flow-cytometry.jpg "Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)")
Key features and details
- Rat monoclonal [YTS169.4] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
概述
-
产品名称
Anti-CD8 alpha抗体[YTS169.4]
参阅全部 CD8 alpha 一抗 -
描述
大鼠单克隆抗体[YTS169.4] to CD8 alpha -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Flow Cyt: Mouse spleen cells.
-
常规说明
ab22378 is not expected to work in standard western blots, under denaturing conditions, or in IHC on formalin-fixed tissue. To our knowledge, ab22378 will only stain frozen sections or fresh cells, and will only immunoprecipitate the antigen from cell lysates in mild detergents such as NP40 or Triton-X100.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
YTS169.4 -
同种型
IgG2b -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22378于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 0.1-1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
-
别名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
图片
-
Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)
Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 µg/mL, detected with Alexa Fluor® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.
-
![Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)](https://www.abcam.cn/ps/products/22/ab22378/Images/ab22378-199649-anti-cd8-antibody-yts1694-flow-cytometry.jpg)
Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)Overlay histogram showing mouse spleen cells (C57BL/6) labeling CD8 alpha with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (96)
ab22378 被引用在 96 文献中.
- Chen J et al. MIF inhibition alleviates vitiligo progression by suppressing CD8+ T cell activation and proliferation. J Pathol 260:84-96 (2023). PubMed: 36852981
- Snipstad S et al. Characterization of immune cell populations in syngeneic murine tumor models. Cancer Med 12:11589-11601 (2023). PubMed: 36912188
- Mukai R et al. Micro-abnormalities in the retina and choroid induced by anti-CTLA4 treatment. Sci Rep 13:6309 (2023). PubMed: 37072549
- Shon WJ et al. Gut taste receptor type 1 member 3 is an intrinsic regulator of Western diet-induced intestinal inflammation. BMC Med 21:165 (2023). PubMed: 37118698
- Wang K et al. Gas therapy potentiates aggregation-induced emission luminogen-based photoimmunotherapy of poorly immunogenic tumors through cGAS-STING pathway activation. Nat Commun 14:2950 (2023). PubMed: 37221157