AB233995

Anti-CD8 alpha抗体[C8/468]

Name: CD8 alpha抗体[C8/468] (ab233995)| Abcam中文官网
Brand: Abcam Limited
SKU: AB233995
Price: 4748 CNY
Availability: InStock

Anti-CD8 alpha antibody [C8/468]

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(1 Publication)

Mouse Monoclonal CD8 alpha antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD8A.

查看别名

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468] (AB233995)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/468] (AB233995)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab233995 at 1 μg/ml in immunohistochemical analysis.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

C8/468

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human CD8A.

P01732

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

纯化说明

Purified from Bioreactor Concentrate by Protein A/G.

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD8A

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