重组Anti-CD8 alpha抗体[BLR044F] - BSA free (ab272093)
![Western blot - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)](https://www.abcam.cn/ps/products/272/ab272093/Images/ab272093-468325-ab243900-anti-cd8-alpha-beta-antibody-western-blot.jpg "Western blot - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR044F] to CD8 alpha - BSA free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD8 alpha抗体[BLR044F] - BSA free
参阅全部 CD8 alpha 一抗 -
描述
兔单克隆抗体[BLR044F] to CD8 alpha - BSA free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CD8 alpha aa 185-235 (C terminal). The exact sequence is proprietary. NP_001759.3 and Gene ID 925.
Database link: P01732 -
常规说明
ab272093 is the BSA-free version of ab243900.
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline -
Concentration information loading... -
纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR044F -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272093于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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别名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
图片
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Western blot - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)All lanes : Anti-CD8 alpha antibody [BLR044F] (ab243900) at 1/1000 dilution
Lane 1 : CD4+ T cell whole cell lysate
Lane 2 : CD8+ T cell whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : MOLT-4 whole cell lysate
Lane 5 : RPMI 8226 whole cell lysate
Lane 6 : MJ whole cell lysate
Lane 7 : C5/MJ whole cell lysate
Lane 8 : KG-1 whole cell lysate
Lane 9 : SR whole cell lysate
Lane 10 : K562 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgGDetection: Chemiluminescence.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243900).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)](https://www.abcam.cn/ps/products/272/ab272093/Images/ab272093-1-ab243900334392anticd8antibodyblr044fimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab243900 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243900).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)](https://www.abcam.cn/ps/products/272/ab272093/Images/ab272093-468326-ab243900-anti-cd8-alpha-beta-antibody-human-melanoma-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243900).
Formalin-fixed, paraffin-embedded human melanoma tissue stained for CD8 alpha (red) using ab243900 at 1/100 dilution in immunohistochemical analysis. A conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)](https://www.abcam.cn/ps/products/272/ab272093/Images/ab272093-468327-ab243900-anti-cd8-alpha-beta-antibody-human-breast-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243900).
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD8 alpha (red) using ab243900 at 1/250 dilution in immunohistochemical analysis. A conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).
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![Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)](https://www.abcam.cn/ps/products/272/ab272093/Images/ab272093-2-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [BLR044F] - BSA free (ab272093)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272093 尚未被引用在任何文献中。