Anti-CD8 alpha抗体(ab4055)
Key features and details
- Rabbit polyclonal to CD8 alpha
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD8 alpha抗体
参阅全部 CD8 alpha 一抗 -
描述
兔多克隆抗体to CD8 alpha -
宿主
Rabbit -
特异性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab178089.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rhesus monkey
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (9) |
1/200.
|
| 说明 |
|---|
|
IHC-P
1/200. |
靶标
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功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 925 Human
- Entrez Gene: 698329 Rhesus monkey
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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别名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (ab4055)This image is courtesy of an Abreview submitted by Carl Hobbs.
ab4055 staining CD8 alpha in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with the primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (ab4055)This image is courtesy of an Abreview submitted by R Berahovichab4055 staining CD8 alpha in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (ab4055)ab4055 at 1/200 staining CD8 alpha in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (ab4055)This image is courtesy of an Abreview submitted by Miss Jill Montgomeryab4055 at 1/500 dilution staining CD8 alpha from human PBMC (Peripheral blood mononuclear cells) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary.
数据表及文件
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SDS download
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Datasheet download
文献 (184)
ab4055 被引用在 184 文献中.
- Wang Y et al. Muramyl dipeptide CD10 monoclonal antibody immunoconjugates inhibited acute leukemia in nude mice. Biosci Rep 43:N/A (2023). PubMed: 37039042
- Cheng M et al. The predictive value of CD4, CD8, and C-reactive protein in the prognosis of schistosomal and non-schistosomal colorectal cancer. BMC Gastroenterol 23:194 (2023). PubMed: 37277702
- Hordeaux J et al. Immune transgene-dependent myocarditis in macaques after systemic administration of adeno-associated virus expressing human acid alpha-glucosidase. Front Immunol 14:1094279 (2023). PubMed: 37033976
- Sato S et al. Bone marrow adipocytes induce cancer-associated fibroblasts and immune evasion, enhancing invasion and drug resistance. Cancer Sci 114:2674-2688 (2023). PubMed: 36916999
- Chimbetete T et al. Skin infiltrating T-cell profile of drug reaction with eosinophilia and systemic symptoms (DRESS) reactions among HIV-infected patients. Front Med (Lausanne) 10:1118527 (2023). PubMed: 37215719