AB4055

Anti-CD8 alpha 抗体

Name: CD8 alpha抗体 (ab4055)| Abcam中文官网
Brand: Abcam Limited
SKU: AB4055
Price: 4429 CNY
Availability: InStock
Rating: 4 (14 reviews)

Anti-CD8 alpha antibody

(14 Reviews)

(243 Publications)

Anti-CD8 alpha antibody (ab4055) is a rabbit polyclonal antibody detecting CD8 alpha in IHC-P. Suitable for Human.

- Over 180 publications
- Trusted since 2003

查看别名

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

IHC-P

AbReview60142****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 staining CD8 alpha in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with the primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 at 1/200 staining CD8 alpha in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

IHC-P

AbReview6718****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 at 1/500 dilution staining CD8 alpha from human PBMC (Peripheral blood mononuclear cells) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Miss Jill Montgomery

IHC-P

AbReview34984****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody (AB4055)

ab4055 staining CD8 alpha in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 5 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by R Berahovich

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab178089.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Product Specifications

Anti-CD8 alpha antibody (ab4055) is a rabbit polyclonal antibody and is validated for use in IHC-P in human samples.
Anti-CD8 alpha antibody (ab4055) specifically detects CD8 alpha (UniProt ID: P01732; Molecular weight: 24kDa) and is sold in 500 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CD8 alpha antibody (ab4055) has high sensitivity and specificity.
Anti-CD8 alpha antibody (ab4055) has been cited over 184 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD8 alpha antibody (ab4055) has 14 independent reviews from customers.

Target Information

CD8 alpha is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR), primarily found on cytotoxic T cells. It plays a crucial role in T cell signaling and cytotoxic activity by binding to MHC class I molecules, aiding in the immune response against infected or malignant cells. CD8 alpha is associated with several diseases, particularly those involving immune system dysfunction.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.

Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD8A

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文献 (243)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113310 PubMed40894910

2025

MEK inhibition prevents human skin graft rejection by promoting CD8TCF1 over CD8 effector T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christine Chauveau,Veronique Nerriere-Daguin,Maeva Fourny,Cynthia Fourgeux,Thibaut Larcher,Laurence Delbos,Martin Braud,Lucas Brusselle,Olivia Rousseau,Jeremie Poschmann,Julien Verdier,Fabienne Haspot,Gilles Blancho,Simon Ville

ImmunoTargets and therapy 14:901-930 PubMed40894302

2025

Ultrasound-Mediated Non-Specific Splenic Immunopotentiation to Elicit Broad-Spectrum Anti-Neoplastic Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Guihu Wang,Senyang Li,Qian Wang,Wenjuan Li,Heyuan Liu,Yingxue Liang,Zhe Zhou,Xinrui He,Wenlei Guo,Jianing Yuan,Yichao Chai,Jing Geng,Zongfang Li

Journal of tissue engineering 16:20417314251360755 PubMed40792201

2025

Transplantation of engineered vascularized lymphatic tissue using LEC and in vivo AV loop model to enhance lymphangiogenesis and restore lymphatic drainage in a lymphadenectomy rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Gina A Mackert,Hui-Yi Hsiao,Yung-Chun Chang,Robin T Wu,Rushil R Dang,Richard Tee,Jung-Ju Huang

Cell death & disease 16:584 PubMed40750787

2025

CHK1 inhibition increases the therapeutic response to radiotherapy via antitumor immunity in ARID1A-deficient colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jhen-Yu Chen,Tao-Wei Ke,Shu-Fen Chiang,Wei-Ze Hong,Hsin-Yu Chang,Ji-An Liang,Yuan-Yao Tsai,Chi-Hsien Huang,William Tzu-Liang Chen,K S Clifford Chao,Kevin Chih-Yang Huang

The Journal of clinical investigation 135: PubMed40311102

2025

Tebentafusp elicits on-target cutaneous immune responses driven by cytotoxic T cells in uveal melanoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon Staeger,Aizhan Tastanova,Adhideb Ghosh,Nicola Winkelbeiner,Prachi Shukla,Isabel Kolm,Patrick Turko,Adel Benlahrech,Jane Harper,Anna Broomfield,Antonio Camera,Marianna Ambrosio,Veronika Haunerdinger,Phil F Cheng,Egle Ramelyte,James Pham,Stefanie Kreutmair,Burkhard Becher,Mitchell P Levesque,Reinhard Dummer,Barbara Meier-Schiesser

NPJ vaccines 10:80 PubMed40258806

2025

Targeting B7-H3 enhances the efficacy of neoantigen-based cancer vaccine in combination with radiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Tao-Wei Ke,Chia-Yi Chen,William Tzu-Liang Chen,Yuan-Yao Tsai,Shu-Fen Chiang,Chi-Hsien Huang,Yu-Sen Lin,Te-Hong Chen,Tsung-Wei Chen,Ji-An Liang,K S Clifford Chao,Kevin Chih-Yang Huang

Frontiers in endocrinology 16:1502117 PubMed40130164

2025

Wnt/β-catenin pathway activation is associated with glucocorticoid secretion in adrenocortical carcinoma, but not directly with immune cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Maier,Laura-Sophie Landwehr,Alexandra Triebig,Stefan Kircher,Marc P Schauer,Thomas Knösel,Silviu Sbiera,Paul Schwarzlmueller,Petra Zimmermann,Martin Reincke,Isabel Weigand,Martin Fassnacht,Matthias Kroiss

Molecular therapy. Methods & clinical development 33:101409 PubMed40104151

2025

Immune-driven gene expression loss following intramuscular AAV delivery to non-human primates is only transient.

Applications

Unspecified application

Species

Unspecified reactive species

Malo Journou,Marie Devaux,Nicolas Jaulin,Virginie Pichard,Mercedes Segovia,Aurélie Moreau,Johanne Le Duff,Maria Cristina Cuturi,Mickaël Guilbaud,Oumeya Adjali

Cancer medicine 14:e70658 PubMed40062505

2025

Radiation-Enhanced AF1q Moves Center Stage as a Key Driver to Favorable Tumor Stage in Rectal Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth S Gruber,Georg Oberhuber,Elisabeth Gurnhofer,Robert Eferl,Gerald Timelthaler,Béla Teleky,D I Dietmar Georg,Joachim Widder,William Tse,Lukas Kenner

In vivo (Athens, Greece) 39:691-701 PubMed40010956

2025

Prognostic Significance of STK11/LKB1 Expression and Its Role in the Tumor Microenvironment of Colorectal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Heng Lee,Chun-Fan Yang,Yih-Farng Liou,Kevin Chih-Yang Huang,K S Clifford Chao,Shu-Fen Chiang

View all publications

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