重组Anti-CD79b抗体[EPR6860] (ab134103)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6860] to CD79b
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-CD79b抗体[EPR6860]
参阅全部 CD79b 一抗 -
描述
兔单克隆抗体[EPR6860] to CD79b -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Daudi, Namalwa, Ramos, and Raji cell lysates, Ramos cells Flow cyto: Human PBMCs
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.90 x 10 -9 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6860 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 35-40 kDa (predicted molecular weight: 26 kDa).
|
|
Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/250 - 1/500.
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 35-40 kDa (predicted molecular weight: 26 kDa). |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250 - 1/500. |
靶标
-
功能
Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. -
组织特异性
B-cells. -
疾病相关
Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
翻译后修饰
Phosphorylated on tyrosine upon B-cell activation. -
细胞定位
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
-
数据库链接
- Entrez Gene: 974 Human
- Omim: 147245 Human
- SwissProt: P40259 Human
- Unigene: 89575 Human
-
别名
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
see all
图片
-
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab134103 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab134103 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 1.0 μg/ml (1/2300)) for 30min on ice. The cells were simultaneously stained with CD19.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
-
All lanes : Anti-CD79b antibody [EPR6860] (ab134103) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD79B CRISPR-Cas9 edited Raji cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD79b antibody [EPR6860] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134103 was shown to bind specifically to CD79b. A band was observed at 39 kDa in wild-type Raji cell lysates with no signal observed at this size in CD79B CRISPR-Cas9 edited cell line ab273839 (CRISPR-Cas9 edited cell lysate ab273793). The band observed in the CRISPR-Cas9 edited lysate lane below 39 kDa is likely to represent a truncated form of CD79b. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and CD79B CRISPR-Cas9 edited Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
All lanes : Anti-CD79b antibody [EPR6860] (ab134103) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Namalwa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 35-40 kDa why is the actual band size different from the predicted? -
Flow Cytometry analysis of Raji cells labeling CD79b with ab134103 at 1/20 dilution (red). A rabbit IgG was used as negative control (green).
-
Immunofluorescence analysis of CD79b in Ramos cells, using ab134103 antibody at a dilution of 1/250.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab134103 被引用在 2 文献中.
- Bullerwell CE et al. EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex. Sci Rep 11:1537 (2021). PubMed: 33452395
- Yoo EJ et al. Identification of a secondary promoter within the human B cell receptor component gene hCD79b. J Biol Chem 288:18353-65 (2013). WB . PubMed: 23649625