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RabMAb

Anti-CD79a抗体[SP18] (ab16698)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)
  • Flow Cytometry (Intracellular) - Anti-CD79a antibody [SP18] (ab16698)

Key features and details

  • Rabbit monoclonal [SP18] to CD79a
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-CD79a抗体[SP18]
    参阅全部 CD79a 一抗

  • 描述

    兔单克隆抗体[SP18] to CD79a

  • 宿主

    Rabbit

  • 特异性

    ab16698 recognises CD79a. We have data to indicate that this antibody may not cross react with Opossum. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    This antibody does not react with mouse species for Flow Cytometry application.

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Monkey

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human tonsil tissue. Flow Cyt (Intra): Jurkat cells.

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab16698于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

  • 组织特异性

    B-cells.

  • 疾病相关

    Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

  • 序列相似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • 翻译后修饰

    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

  • 细胞定位

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • B lymphocyte-specific MB1 protein antibody
    • B-cell antigen receptor complex-associated protein alpha chain antibody
    • CD 79a antibody
    • CD79a antibody
    • CD79a antigen (immunoglobulin-associated alpha) antibody
    • CD79A antigen antibody
    • CD79a molecule, immunoglobulin-associated alpha antibody
    • CD79A_HUMAN antibody
    • Ig alpha antibody
    • Ig-alpha antibody
    • IGA antibody
    • IgM-alpha antibody
    • Immunoglobulin-associated alpha antibody
    • Ly54 antibody
    • MB-1 membrane glycoprotein antibody
    • MB1 antibody
    • Membrane-bound immunoglobulin-associated protein antibody
    • Surface IgM-associated protein antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)
    Ab16698, at a dilution of 1/100, staining formalin fixed paraffin embedded human tonsil tissue sections by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [SP18] (ab16698)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD79a with ab16698 at 1/400 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Flow Cytometry (Intracellular) - Anti-CD79a antibody [SP18] (ab16698)
    Flow Cytometry (Intracellular) - Anti-CD79a antibody [SP18] (ab16698)

    Flow cytometric analysis of rabbit anti-CD79a (SP18) antibody ab16698 (1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

文献 (1)

发表研究结果有使用 ab16698？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab16698 被引用在 1 文献中.

  • Zhang AW  et al. Interfaces of Malignant and Immunologic Clonal Dynamics in Ovarian Cancer. Cell 173:1755-1769.e22 (2018). PubMed: 29754820

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Flow Cytometry abreview for Anti-CD79a antibody [SP18]

 Poor
Abreviews
abreview image
Application
Flow Cytometry
Sample
Pig Cell (PBMCs)
Permeabilization
No
Gating Strategy
B cells
Specification
PBMCs
Preparation
Cell harvesting/tissue preparation method: PBMCs after percoll gradient separation
Sample buffer: PBS + 5% FBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Feb 02 2023

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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