Anti-CD79a抗体[HM57] (ab62650)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [HM57] (ab62650)](https://www.abcam.cn/ps/products/62/ab62650/Images/ab62650-331745-anti-cd79a-antibody-hm57-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [HM57] (ab62650)")
Key features and details
- Mouse monoclonal [HM57] to CD79a
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD79a抗体[HM57]
参阅全部 CD79a 一抗 -
描述
小鼠单克隆抗体[HM57] to CD79a -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
ISRGLQGTYQDVGSL
, corresponding to amino acids 202-216 of Human CD79a -
阳性对照
- Human tonsil tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
克隆
单克隆 -
克隆编号
HM57 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62650于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
| 说明 |
|---|
应用说明
Flow Cyt: 1/10 - 1/50. Use 10µl to label 1x106 cells in 100µl. Membrane permeabilisation is required for this application.
IHC-P: 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells. -
组织特异性
B-cells. -
疾病相关
Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
翻译后修饰
Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation. -
细胞定位
Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 973 Human
- Omim: 112205 Human
- SwissProt: P11912 Human
- Unigene: 631567 Human
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别名
- B lymphocyte-specific MB1 protein antibody
- B-cell antigen receptor complex-associated protein alpha chain antibody
- CD 79a antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [HM57] (ab62650)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining CD79a with ab62650.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab62650 被引用在 4 文献中.
- Chludzinski E et al. Case Report: Primary Diffuse Leptomeningeal Oligodendrogliomatosis in a Young Adult Cat. Front Vet Sci 8:795126 (2021). PubMed: 34977226
- Dudek K et al. Analysis of the Leukocyte Response in Calves Suffered from Mycoplasma bovis Pneumonia. Pathogens 9:N/A (2020). PubMed: 32456293
- Boettcher AN et al. Novel Engraftment and T Cell Differentiation of Human Hematopoietic Cells in ART-/-IL2RG-/Y SCID Pigs. Front Immunol 11:100 (2020). PubMed: 32117254
- Döring AK et al. Cruciate ligament degeneration and stifle joint synovitis in 56 dogs with intact cranial cruciate ligaments: Correlation of histological findings and numbers and phenotypes of inflammatory cells with age, body weight and breed. Vet Immunol Immunopathol 196:5-13 (2018). PubMed: 29695325