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AB321802

Anti-CD79a antibody [EPR29061-505] - BSA and Azide free

Anti-CD79a antibody [EPR29061-505] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD79A antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples.

查看别名

CD79a, Iga, Mb-1, Cd79a, B-cell antigen receptor complex-associated protein alpha chain, Ig-alpha, MB-1 membrane glycoprotein, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

5 Images
Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)

This data was developed using ab321801, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse PBMC cells labelling CD79a with ab321801 at 1/100 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse PBMC are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.

Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)

This data was developed using ab321801, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of EL4 cells (mouse lymphoma T lymphocyte, Left) / WEHI-231 cells (mouse B cell lymphoma B lymphocyte, Right). Labelling CD79a with ab321801 at 1/100 dilution (1 μg) (Red), compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) or unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : EL4
Gated on viable cell.

Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)

This data was developed using ab321801, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (Mouse primary peripheral blood mononuclear cell ) cells labelling CD79a with ab321801 at 1/500 (1.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic and membranous staining in subsets of CD3 negative mouse PBMC. (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-mouse CD3 rat monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)

This data was developed using ab321801, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse PBMC cells labelling CD79a with ab321801 at 1/100 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse PBMC are co-stained with CD19 conjugated Alexa Fluor®647.
Gated on viable cell.

Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EPR29061-505] - BSA and Azide free (AB321802)

This data was developed using ab321801, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD79a with ab321801 at 1/500 (1.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ab150081 1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic and membranous staining in WEHI-231 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : EL4.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ab150081 1000 2ug/ml dilution.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29061-505

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

ab321802 is the carrier-free version of ab321801.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: 100% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD79a also known as Ig-alpha or MB-1 is a component of the B cell antigen receptor (BCR) complex. It is a transmembrane protein with a molecular mass of approximately 47 kDa. CD79a is part of a heterodimer with CD79b and is mainly expressed on the surface of B lymphocytes. This protein plays a critical mechanical role in signaling transduction through the BCR by associating with membrane-bound immunoglobulin molecules helping to initiate the immune response upon antigen binding.
Biological function summary

This protein acts as an essential mediator in the immune system. CD79a together with CD79b forms part of the BCR complex critical for B cell development and maturation. The BCR complex activates signaling cascades that influence B cell fate decisions such as proliferation differentiation and apoptosis. By transmitting signals from the BCR CD79a helps regulate these cellular functions necessary for efficient immune defense.

Pathways

CD79a plays a pivotal role in the B cell receptor signaling pathway. This pathway involves signaling molecules such as spleen tyrosine kinase (SYK) and phosphoinositide 3-kinase (PI3K) which are activated upon BCR engagement. CD79a acts in concert with other proteins including CD79b and the IgM receptor facilitating downstream signaling cascades that ensure proper B cell responses. Its role is important for linking extracellular antigen recognition to intracellular signaling events.

CD79a is significant in conditions like B cell lymphomas and certain immunodeficiencies. Abnormal expression or mutations in CD79a are associated with some types of non-Hodgkin lymphoma. The protein's interaction with CD79b is particularly relevant as alterations in these proteins' function or expression can impact B cell development and lead to pathogenesis in lymphoproliferative disorders. Consequently CD79a serves as an important biomarker and potential therapeutic target in these diseases.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
See full target information Cd79a
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