重组Anti-CD73抗体[AD2] (ab130451)
![Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)](https://www.abcam.cn/ps/products/130/ab130451/Images/ab130451-412385-anti-cd73-antibody-ad2-flow-cytometry-pbmc-human1.JPG "Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AD2] to CD73
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD73抗体[AD2]
参阅全部 CD73 一抗 -
描述
小鼠单克隆抗体[AD2] to CD73 -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
The details of the immunogen for this antibody are not available.
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阳性对照
- Flow Cyt: Human PBMCs.
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常规说明
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AD2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- APC Anti-CD73 antibody [AD2], prediluted (ab155378)
- PE Anti-CD73 antibody [AD2] (ab157335)
- FITC Anti-CD73 antibody [AD2] (ab239246)
- Anti-CD73 antibody [AD2] - BSA and Azide free (ab239603)
- Alexa Fluor® 647 Anti-CD73 antibody [AD2] (ab243083)
- Alexa Fluor® 488 Anti-CD73 antibody [AD2] (ab252554)
- PE/Cy7® Anti-CD73 antibody [AD2] (ab253253)
- PE/Cy5® Anti-CD73 antibody [AD2] (ab253267)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use a concentration of 1 - 4 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4907 Human
- Omim: 129190 Human
- SwissProt: P21589 Human
- Unigene: 153952 Human
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别名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
图片
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Flow Cytometry - Anti-CD73 antibody [AD2] (ab130451)
Flow cytometric analysis of human PBMC cells labeling CD73 with ab130451 at 1/500 dilution (Right) compared with an isotype control (Left).
Cells were blocked with 20 μg/mL human IgG for 15 minutes on ice. Primary antibody incubation for 30 minutes on ice.
Goat Anti-Mouse IgG H&L (AlexaFluor® 488) (ab150113) was used as secondary antibody for 30 minutes on ice in the dark. Cells were also stained with Anti-Mouse CD19 conjugated to AlexaFluor® 647 for 30 minutes on ice in the dark. Added 7-AAD in 1X PBS and incubated for 5 minutes.
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![Anti-CD73 antibody [AD2] (ab130451)](https://www.abcam.cn/ps/products/130/ab130451/Images/ab130451-3-benefits-of-recombinant-antibodies.png)
Anti-CD73 antibody [AD2] (ab130451)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab130451 被引用在 2 文献中.
- Doshmanziari M et al. Mesenchymal stem cells act as stimulators of neurogenesis and synaptic function in a rat model of Alzheimer's disease. Heliyon 7:e07996 (2021). PubMed: 34589625
- Borrione P et al. CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression. J Immunol 162:6238-46 (1999). PubMed: 10229870