Anti-CD73抗体[7G2] (ab54217)
![Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)](https://www.abcam.cn/ps/products/54/ab54217/Images/ab54217-459737-AntiCD73-antibody-immunocytochemsitry-u87mg-cells.jpg "Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)")
Key features and details
- Mouse monoclonal [7G2] to CD73
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-CD73抗体[7G2]
参阅全部 CD73 一抗 -
描述
小鼠单克隆抗体[7G2] to CD73 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICCmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Full length purified Human placental CD73.
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阳性对照
- Flow Cyt: Human peripheral blood lymphocytes. ICC: U-87 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
7G2 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use a concentration of 1 - 2 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| ICC | (1) |
1/100.
|
| 说明 |
|---|
|
Flow Cyt
Use a concentration of 1 - 2 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC
1/100. |
应用说明
Is unsuitable for WB.
靶标
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功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4907 Human
- Omim: 129190 Human
- SwissProt: P21589 Human
- Unigene: 153952 Human
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别名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
图片
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Immunocytochemistry - Anti-CD73 antibody [7G2] (ab54217)Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ab54217 at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with a Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate, (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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![Flow Cytometry - Anti-CD73 antibody [7G2] (ab54217)](https://www.abcam.cn/ps/products/54/ab54217/Images/ab54217-67000-anti-cd73-antibody-7g2-flow-cytometry.jpg)
Flow Cytometry - Anti-CD73 antibody [7G2] (ab54217)ab54217 staining human peripheral blood lymphocytes by FACS analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (30)
ab54217 被引用在 30 文献中.
- Zhang Y et al. Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells. Commun Biol 6:476 (2023). PubMed: 37127734
- Perez JE et al. Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells. J Nanobiotechnology 20:282 (2022). PubMed: 35710420
- Sikora M et al. Bone marrow stromal cells (BMSCs CD45- /CD44+ /CD73+ /CD90+ ) isolated from osteoporotic mice SAM/P6 as a novel model for osteoporosis investigation. J Cell Mol Med 25:6634-6651 (2021). PubMed: 34075722
- Russo E et al. Energy Metabolism Analysis of Three Different Mesenchymal Stem Cell Populations of Umbilical Cord Under Normal and Pathologic Conditions. Stem Cell Rev Rep 16:585-595 (2020). PubMed: 32185666
- Ou X et al. Activation of SIRT1 promotes cartilage differentiation and reduces apoptosis of nucleus pulposus mesenchymal stem cells via the MCP1/CCR2 axis in subjects with intervertebral disc degeneration. Int J Mol Med 46:1074-1084 (2020). PubMed: 32705163