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Immunology Cell Type Markers CD Myeloid Cells
重组

重组Anti-CD68抗体[KP1] (ab955)

  • Datasheet
  • SDS
Reviews (46)Q&A (2)References (437)

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Western blot - Anti-CD68 antibody [KP1] (ab955)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
  • Anti-CD68 antibody [KP1] (ab955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [KP1] to CD68
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free

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概述

  • 产品名称

    Anti-CD68抗体[KP1]
    参阅全部 CD68 一抗
  • 描述

    小鼠单克隆抗体[KP1] to CD68
  • 宿主

    Mouse
  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Rabbit
  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: human tonsil and liver tissues; ICC/IF: THP-1 cells; WB: Human spleen lysate.
  • 常规说明

    Anti CD68 antibody (ab955) is reccomended for IHC on human samples but is not reccomended for mouse & rat samples. 

    This product has switched from a hybridoma to recombinant production method on 21st September 2020. The concentration listed relates to the purified version, please contact Scientific Support for unpurified concentration. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    KP1
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Neuroscience
    • Development

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD68 antibody [KP1] (ab222914)
    • Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
    • Alexa Fluor® 555 Anti-CD68 antibody [KP1] (ab279323)
    • Anti-CD68 antibody [KP1], prediluted (ab845)
  • Antibody Diluent

    • Universal antibody diluent (ab79995)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)
  • Related Products

    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab955于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (5)
1/50.
WB (1)
1/1000. Predicted molecular weight: 37 kDa.
IHC-P (29)
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
ICC/IF
1/50.
WB
1/1000. Predicted molecular weight: 37 kDa.
IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性

    Belongs to the LAMP family.
  • 翻译后修饰

    N- and O-glycosylated.
  • 细胞定位

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Target information above from: UniProt accession P34810 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 968 Human
    • Entrez Gene: 12514 Mouse
    • Entrez Gene: 287435 Rat
    • Omim: 153634 Human
    • SwissProt: P34810 Human
    • SwissProt: P31996 Mouse
    • Unigene: 647419 Human
    • Unigene: 15819 Mouse
    • 别名

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    图片

    • Western blot - Anti-CD68 antibody [KP1] (ab955)
      Western blot - Anti-CD68 antibody [KP1] (ab955)
      Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution + Human spleen lysate at 20 µg

      Secondary
      Goat anti-Mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution

      Predicted band size: 37 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      The observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)
      Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)

      Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD28 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).

      Confocal image showing cytoplasmic staining in THP-1 cells

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.

      ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreview

      ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

      See Abreview

    • Anti-CD68 antibody [KP1] (ab955)
      Anti-CD68 antibody [KP1] (ab955)

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (437)

    发表研究结果有使用 ab955?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab955 被引用在 437 文献中.

    • Hou C  et al. Mono-macrophage-Derived MANF Protects Against Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting Inflammation and Renal M1 Macrophages. Inflammation 44:693-703 (2021). PubMed: 33145627
    • McGinley LM  et al. Magnetic resonance imaging of human neural stem cells in rodent and primate brain. Stem Cells Transl Med 10:83-97 (2021). PubMed: 32841522
    • Lee SE  et al. Intravenous allogeneic umbilical cord blood-derived mesenchymal stem cell therapy in recessive dystrophic epidermolysis bullosa patients. JCI Insight 6:N/A (2021). PubMed: 33491668
    • Ni XQ  et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY) 13:5164-5184 (2021). PubMed: 33535178
    • Chen KT  et al. Neuronavigation-guided focused ultrasound for transcranial blood-brain barrier opening and immunostimulation in brain tumors. Sci Adv 7:N/A (2021). PubMed: 33547073
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-10 of 48 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD68 antibody [KP1]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Vein graft)
    Specification
    Vein graft
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Linxiang Bi

    Verified customer

    提交于 May 19 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 17 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Rabbit liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 citrate buffer
    Permeabilization
    No
    Specification
    Rabbit liver
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 10 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 24 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph:6.0
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 08 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD68 antibody [KP1]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (HT29 tumors taken out Balb/C nude mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph6
    Permeabilization
    No
    Specification
    HT29 tumors taken out Balb/C nude mice
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (CT26 tumor taken out Balb/C mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph6
    Permeabilization
    No
    Specification
    CT26 tumor taken out Balb/C mice
    Blocking step
    Immunofluorescence Blocking Buffer #12411 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 16 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD68 antibody [KP1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Peritoneal aspirate)
    Permeabilization
    No
    Specification
    Peritoneal aspirate
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    提交于 Oct 20 2019

    1-10 of 48 Abreviews or Q&A

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