重组Anti-CD68抗体[KP1] (ab955)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KP1] to CD68
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD68抗体[KP1]
参阅全部 CD68 一抗 -
描述
小鼠单克隆抗体[KP1] to CD68 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: human tonsil and liver tissues; ICC/IF: THP-1 cells; WB: Human spleen lysate, THP-1 whole cell lysate IMC: Human tonsil tissue
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常规说明
Anti CD68 antibody (ab955) is recommended for IHC on human samples but is not recommended for mouse & rat samples.
This product has switched from a hybridoma to recombinant production method on 21st September 2020. The concentration listed relates to the purified version, please contact Scientific Support for unpurified concentration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
KP1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (5) |
1/50.
|
WB | (1) |
1/1000. Predicted molecular weight: 37 kDa.
|
IHC-P | (30) |
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/50. |
WB
1/1000. Predicted molecular weight: 37 kDa. |
IHC-P
1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
组织特异性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
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别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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Lanes 1 & 3 : Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution
Lanes 2 & 4 : Anti-CD68 antibody [RM1031] (ab303565) at 1/1000 dilution
Lanes 1-2 : Human spleen tissue lysate
Lanes 3-4 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST
Exposure time: Lane1: 60 seconds; Lane2: 5 seconds; Lane3: 180 seconds; Lane4: 7 seconds
ab303565 works better than ab955 in western blot testing.
We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results when using ab955 in western blot.
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Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD68 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in THP-1 cells
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.
ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreview
ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (618)
ab955 被引用在 618 文献中.
- Hu X et al. Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques. Nat Biotechnol 42:413-423 (2024). PubMed: 37156915
- Li Y et al. Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD. Front Immunol 14:1162087 (2023). PubMed: 37215140
- Zhu F et al. β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals. Immun Inflamm Dis 11:e876 (2023). PubMed: 37249285
- Zhang Y et al. Janus porous polylactic acid membranes with versatile metal-phenolic interface for biomimetic periodontal bone regeneration. NPJ Regen Med 8:28 (2023). PubMed: 37270633
- Jing L et al. A subpopulation of CD146+ macrophages enhances antitumor immunity by activating the NLRP3 inflammasome. Cell Mol Immunol 20:908-923 (2023). PubMed: 37308559