Anti-CD68抗体[ED1] (ab31630)
![Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)](https://www.abcam.cn/ps/products/31/ab31630/Images/ab31630-251826-anti-cd68-antibody-ed1-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)")
Key features and details
- Mouse monoclonal [ED1] to CD68
- Suitable for: Flow Cyt (Intra)
- Reacts with: Rat
- Isotype: IgG1
概述
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产品名称
Anti-CD68抗体[ED1]
参阅全部 CD68 一抗 -
描述
小鼠单克隆抗体[ED1] to CD68 -
宿主
Mouse -
特异性
Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68. -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Rat -
免疫原
Tissue, cells or virus corresponding to Rat CD68. Rat spleen cell.
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阳性对照
- Flow Cyt (Intra): Rat peritoneal macrophages.
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常规说明
This product should be stored undiluted.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
ED1 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
组织特异性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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数据库链接
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别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)
Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm.
数据表及文件
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SDS download
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Datasheet download
文献 (403)
ab31630 被引用在 403 文献中.
- Wei J et al. Phenolic acids from Prunella vulgaris alleviate cardiac remodeling following myocardial infarction partially by suppressing NLRP3 activation. Phytother Res 38:384-399 (2024). PubMed: 37992723
- Al Aameri RFH et al. Targeting CXCL1 chemokine signaling for treating cisplatin ototoxicity. Front Immunol 14:1125948 (2023). PubMed: 37063917
- Huang Z et al. Macrophage contribution to the survival of transferred expanded skin flap through angiogenesis. Ann Transl Med 11:248 (2023). PubMed: 37082668
- Kim JE et al. Epigallocatechin-3-Gallate Attenuates Leukocyte Infiltration in 67-kDa Laminin Receptor-Dependent and -Independent Pathways in the Rat Frontoparietal Cortex following Status Epilepticus. Antioxidants (Basel) 12:N/A (2023). PubMed: 37107345
- Yamauchi N et al. High-intensity interval training in the form of isometric contraction improves fatigue resistance in dystrophin-deficient muscle. J Physiol 601:2917-2933 (2023). PubMed: 37184335