Anti-CD68抗体(ab125212)
Key features and details
- Rabbit polyclonal to CD68
- Suitable for: WB, IHC-P, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CD68抗体
参阅全部 CD68 一抗 -
描述
兔多克隆抗体to CD68 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse CD68 aa 312-326 (internal sequence). Different from the related rat sequence by one amino acid.
Sequence:AFCITRRRQSTYQPL
Database link: P31996 -
阳性对照
- IHC-P: Rat and mouse liver tissue, Mouse spleen, skin, and brain tissues; IHC-Fr: Rat liver tissue; WB: Wild-type RAW 264.7, Neuro-2a cell lysates, Rat and Mouse spleen tissue lysates. Raw264.7 cell lysate.
-
常规说明
View a monoclonal antibody available to this target, ab31630.
For WB, as CD68 is highly glycosylated, it typically runs between 75-110 kDa depending on the amount of glycosylation in the sample.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, 5.61% Trehalose -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125212于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (9) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 35 kDa.
The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions. |
IHC-P | (33) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
IHC-Fr | (7) |
Use a concentration of 0.5 - 1 µg/ml.
|
说明 |
---|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 35 kDa. The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
靶标
-
功能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
组织特异性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- SwissProt: P31996 Mouse
- Unigene: 15819 Mouse
-
别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
-
All lanes : Anti-CD68 antibody (ab125212) at 0.2 µg/ml
Lane 1 : Wild-type RAW 264.7 cell lysate
Lane 2 : CD68 knockout RAW 264.7 cell lysate
Lane 3 : Mouse spleen cell lysate
Lane 4 : Neuro-2a cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 95-102 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD68 antibody staining at 0.2 µg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab125212 was shown to bind specifically to CD68. A band was observed at 95-102 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in CD68 knockout cell line ab280047 (knockout cell lysate ab280106). To generate this image, wild-type and CD68 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
Paraffin-embedded rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous Abreview.
ab125212 staining CD68 in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA + 10% FCS) for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab125212 overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
-
All lanes : Anti-CD68 antibody (ab125212)
Lane 1 : Rat spleen tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : RAW264.7 cell lysate
Predicted band size: 35 kDa
Additional bands at: 90-100 kDa (possible glycosylated form)Western blot analysis of rat and mouse tissue lysates, labelling CD68 with ab125212
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)
Paraffin-embedded mouse spleen tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)Immunohistochemistry analysis of paraffin-embedded rat liver tissue labeling CD68 with ab125212. Heat mediated antigen retrieval was performed in citrate buffer (pH 6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti- CD68 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)Wes, P.D. PLoS One. 2014 Aug 25;9(8):e106050. doi: 10.1371/journal.pone.0106050. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry of microglial marker in mouse brain
Cortical staining for the microglial marker, CD68 increased as a function of age in rTg4510 animals, but remained unchanged in tTA animals. Scale bar, 200 µm, inset scale bar, 50 µm.
(After Figure 10 A of Wes et al.)
-
Frozen sectioned rat liver tissue stained for CD68 using ab125212 at 1 μg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous Abreview.
ab125212 staining CD68 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody (ab125212)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.
Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (undiluted) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.
数据表及文件
-
Datasheet download
文献 (586)
ab125212 被引用在 586 文献中.
- Su Y et al. Blending with transition metals improves bioresorbable zinc as better medical implants. Bioact Mater 20:243-258 (2023). PubMed: 35702610
- Yuan X et al. Spinal voltage-gated potassium channel Kv1.3 contributes to neuropathic pain via the promotion of microglial M1 polarization and activation of the NLRP3 inflammasome. Eur J Pain 27:289-302 (2023). PubMed: 36440534
- Li Z et al. A fully biodegradable polydioxanone occluder for ventricle septal defect closure. Bioact Mater 24:252-262 (2023). PubMed: 36632501
- Yu Z et al. Icaritin inhibits neuroinflammation in a rat cerebral ischemia model by regulating microglial polarization through the GPER-ERK-NF-κB signaling pathway. Mol Med 28:142 (2022). PubMed: 36447154
- Chen H et al. The E3 ubiquitin ligase WWP2 regulates pro-fibrogenic monocyte infiltration and activity in heart fibrosis. Nat Commun 13:7375 (2022). PubMed: 36450710