重组Anti-CD64抗体[EPR4624] (ab134073)
![Western blot - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.cn/ps/products/134/ab134073/Images/ab134073-483354-anti-cd64-antibody-epr4624-western-blot-wildtype-thp1-fcgr1a-knockout-thp1-u937-hek293.jpg "Western blot - Anti-CD64 antibody [EPR4624] (ab134073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4624] to CD64
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD64抗体[EPR4624]
参阅全部 CD64 一抗 -
描述
兔单克隆抗体[EPR4624] to CD64 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: THP-1 and U-937 whole cell lysates IP: ab134073 + THP-1 whole cell lysate
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常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4624 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
|
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| IP |
1/30.
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| 说明 |
|---|
|
WB
1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa). |
|
IP
1/30. |
靶标
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功能
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
组织特异性
Monocyte/macrophage specific. -
序列相似性
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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数据库链接
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
别名
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
图片
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Western blot - Anti-CD64 antibody [EPR4624] (ab134073)All lanes : Anti-CD64 antibody [EPR4624] (ab134073) at 1/10000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : FCGR1A knockout THP-1 cell lysate
Lane 3 : U937 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD64 antibody [EPR4624] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134073 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image, wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Immunoprecipitation - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.cn/ps/products/134/ab134073/Images/ab134073-5-anti-cd64-antibody-epr4624-immunoprecipitation-thp1-human.jpg)
Immunoprecipitation - Anti-CD64 antibody [EPR4624] (ab134073)Purified ab134073 at 1:30 dilution (2µg) immunoprecipitating CD64 in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg.
Lane 2 (+): ab134073 + THP-1 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134073 in THP-1 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)™(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: Glycosylated form: 64-78kDa; Non-glycosylated form: 39-43kDa. -
![Western blot - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.cn/ps/products/134/ab134073/Images/ab134073-478309-anti-cd64-antibody-epr4624-westernblot-human.jpg)
Western blot - Anti-CD64 antibody [EPR4624] (ab134073)All lanes : Anti-CD64 antibody [EPR4624] (ab134073) at 1/10000 dilution (Purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaGlycosylated form: 64-78kDa; Non-glycosylated form: 39-43kDa
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![Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.cn/ps/products/134/ab134073/Images/ab134073-2-benefits-of-recombinant-antibodies.png)
Anti-CD64 antibody [EPR4624] (ab134073)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab134073 被引用在 3 文献中.
- Li F et al. Jiyuan oridonin A induces differentiation of acute myeloid leukemia cells including leukemic stem-like cells. Front Pharmacol 13:1001552 (2022). PubMed: 36133825
- Hintz HM et al. Simultaneous Engagement of Tumor and Stroma Targeting Antibodies by Engineered NK-92 Cells Expressing CD64 Controls Prostate Cancer Growth. Cancer Immunol Res 9:1270-1282 (2021). PubMed: 34452926
- Duffney PF et al. Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors. J Biol Chem 295:12727-12738 (2020). PubMed: 32690608