Anti-CD64 抗体 [10.1]

• Flow Cyt

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Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Flow Cytometry analysis of human peripheral whole blood labeling CD64 positive monocytes with ab119843 at 4 μg/mL (red-filled).

Negative control : CD64 negative lymphocytes (black-dashed).

• Flow Cyt

Unknown

Flow Cytometry - Anti-CD64 antibody [10.1] (AB119843)

Surface staining of CD64 in human peripheral blood with anti-CD64 (10.1) purified , GAM-APC.