Anti-CD63抗体[TS63] - BSA and Azide free (ab59479)
![Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)](https://www.abcam.cn/ps/products/59/ab59479/Images/CD63-Primary-antibodies-ab59479-2.jpg "Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)")
Key features and details
- Mouse monoclonal [TS63] to CD63 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD63抗体[TS63] - BSA and Azide free
参阅全部 CD63 一抗 -
描述
小鼠单克隆抗体[TS63] to CD63 - BSA and Azide free -
宿主
Mouse -
特异性
Recognises the LIMP antigen, a 30-60 kda (smear) protein. -
经测试应用
适用于: ICC, Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD63. Jurkat and HEL cell lines.
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阳性对照
- ICC: A431 cells; IHC-P: Human Skin Melanoma; Flow Cyt: HL60 cells;
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
无载体
是 -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Sterile-filtered through 0.22 µm and treated to remove endotoxins. -
克隆
单克隆 -
克隆编号
TS63 -
骨髓瘤
P3-X63 Ag8.3 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (1) |
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa).
|
| 说明 |
|---|
|
ICC
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa). |
靶标
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功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
组织特异性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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数据库链接
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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别名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
图片
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Immunocytochemistry - Anti-CD63 antibody [TS63] - BSA and Azide free (ab59479)
ICC image of ab59479 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59479 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [TS63] (ab59479)](https://www.abcam.cn/ps/products/59/ab59479/Images/ab59479-210431-ab59479GR589165Edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [TS63] (ab59479)IHC image of CD63 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59479, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Flow Cytometry - Anti-CD63 antibody [TS63] (ab59479)](https://www.abcam.cn/ps/products/59/ab59479/Images/CD63-Primary-antibodies-ab59479-3.jpg)
Flow Cytometry - Anti-CD63 antibody [TS63] (ab59479)Overlay histogram showing HL60 cells stained with ab59479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59479, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight®488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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Datasheet download
文献 (250)
ab59479 被引用在 250 文献中.
- Wu Q et al. Presence of Intact Hepatitis B Virions in Exosomes. Cell Mol Gastroenterol Hepatol 15:237-259 (2023). PubMed: 36184032
- Toshida K et al. Cancer-associated fibroblasts promote tumor cell growth via miR-493-5p in intrahepatic cholangiocarcinoma. Cancer Sci 114:937-947 (2023). PubMed: 36369960
- Zhang J et al. Surface Engineering of HEK293 Cell-Derived Extracellular Vesicles for Improved Pharmacokinetic Profile and Targeted Delivery of IL-12 for the Treatment of Hepatocellular Carcinoma. Int J Nanomedicine 18:209-223 (2023). PubMed: 36660339
- Karvinen S et al. Extracellular vesicles and high-density lipoproteins: Exercise and oestrogen-responsive small RNA carriers. J Extracell Vesicles 12:e12308 (2023). PubMed: 36739598
- Morimoto Y et al. MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. J Immunother Cancer 11:N/A (2023). PubMed: 36754452
